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山羊抗叙利亚仓鼠IgG H&L (HRP)预吸附二抗(ab7146)

Submit a review Q&A (2)References (2)

Key features and details

  • Goat Anti-Syrian Hamster IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Suitable for: Dot blot, IHC-P, ICC/IF, Immunomicroscopy, WB, IHC-Fr, ELISA

概述

  • 产品名称

    山羊抗叙利亚仓鼠IgG H&L (HRP)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Syrian hamster

  • 经测试应用

    适用于: Dot blot, IHC-P, ICC/IF, Immunomicroscopy, WB, IHC-Fr, ELISAmore details

  • 最小交叉反应


    Chicken, Cow, Goat, Guinea pig, Horse, Human, Mouse, Rabbit, Rat, Sheep more details

  • 免疫原

    hamster IgG whole molecule

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    Horseradish Peroxidase (HRP)

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7146于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Dot blot
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2500.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/15000 - 1/75000.
说明
Dot blot
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2500.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/1000 - 1/5000.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/15000 - 1/75000.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (2)

发表研究结果有使用 ab7146？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7146 被引用在 2 文献中.

  • Meyer M  et al. mRNA-1273 efficacy in a severe COVID-19 model: attenuated activation of pulmonary immune cells after challenge. bioRxiv N/A:N/A (2021). PubMed: 33532780
  • Miezeiewski M  et al. An in vitro culture model to study the dynamics of colonic microbiota in Syrian golden hamsters and their susceptibility to infection with Clostridium difficile. ISME J N/A:N/A (2014). PubMed: 25036923

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

I did try the primary with a different secondary and the results were beautiful.  I also tried dilutions of the secondary as low as 1:50 to see if I could see any staining.  Furthermore I tried the secondary ab7146 at 1:1000 on a Western and got the cleanest (no bands at all) Western blot I have ever seen.  I think that something is wrong with the secondary.  Could you replace it with Goat polyclonal Secondary Antibody to Syrian Hamster IgG - H&L (HRP) (ab6892)?  Or do you suggest something else?  I only suggest this one instead because it has a reference associated with it.    While I have your ear, I have been meaning to email you about another issue.  We received a new lyve-1 antibody ab14947 on Sept 29, 2011 and we have not had any success using our established protocol.  The established protocol was worked up on lot *** and utilized to stain over 100 slides.  When the new lot, ****, the protocol stopped working.  We have tried different dilutions and we still have no stain.  Furthermore, all other reagents are fine as they have been utilized with different primaries and positive results obtained.  Have you received any other complaints about this lot, and is it possible there may be something wrong?  Do you have any of the other lot available?  Can you replace with a new lot?

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Answer

Thank you for the followup. I agree this needs to be replaced. The antibody you have picked as a replacement, ab6892, is available, but it is not pre-adsorbed. (Ab7146 is pre-adsorbed, to remove reactivity with other species of IgG). Will this be acceptable? It is usually only a requirement if one is staining for multiple targets in a single sample with multiple antibodies. There have not been other complaints about ab14917. We have two more vials of lot **** but they may move quickly. Can you please send a few details of your  IHC protocol (fixation, retrieval, diilution) and samples? Given that it was working with lot ****, we expect it would work with the most recent, but if you are working with a species other than mouse, there might be some lot-to-lot variability with respect to cross-reactivity.

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Question

Will the antibody ab7146 cross react with Armenian Hamster IgG?

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Answer

The antibody ab7146 will not work very well with Armenian Hamster IgG and I have been informed that the most suitable antibody is ab5745 (Rabbit polyclonal to Hamster (Armenian) IgG H&L (HRP)) https://www.abcam.com/Rabbit-Armenian-hamster-IgG-HL-HRP-ab5745.html I hope this information will help you, please do not hesitate to contact me if I can be of further assistance,

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