山羊抗叙利亚仓鼠IgG H&L (Alexa Fluor® 488) (ab180063)
Key features and details
- Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488)
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ELISA, ICC/IF, Flow Cyt, IHC-Fr
Related conjugates and formulations
概述
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产品名称
山羊抗叙利亚仓鼠IgG H&L (Alexa Fluor® 488)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Syrian hamster -
经测试应用
适用于: IHC-P, ELISA, ICC/IF, Flow Cyt, IHC-Frmore details -
免疫原
The details of the immunogen for this antibody are not available.
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偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
克隆
多克隆 -
同种型
IgG -
常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180063于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
1/200 - 1/1000.
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| Flow Cyt |
1/2000.
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| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
1/200 - 1/1000. |
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Flow Cyt
1/2000. |
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IHC-Fr
Use at an assay dependent concentration. |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) (ab180063)
IHC image of Podoplanin / gp36 staining in a section of formalin-fixed paraffin-embedded normal mouse lung. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab11936 at 1/250. The secondary antibody (shown in green) was ab180063 at 1/1000 (Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) and counterstained using ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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Alexa Fluor® - Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) (ab180063) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) (ab180063)IHC image of Podoplanin / gp36 staining in a section of formalin-fixed paraffin-embedded normal mouse skin. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab11936 at 1/250. The secondary antibody (shown in green) was ab180063 at 1/500 (Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) and counterstained using ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab180063 被引用在 7 文献中.
- Yu Y et al. Arginase 1 and L-arginine coordinate fetal lung development and the initiation of labor in mice. EMBO Rep 24:e56352 (2023). PubMed: 37291976
- Vankriekelsvenne E et al. Transmembrane protein 119 is neither a specific nor a reliable marker for microglia. Glia 70:1170-1190 (2022). PubMed: 35246882
- Nguyen TM et al. Stretch increases alveolar type 1 cell number in fetal lungs through ROCK-Yap/Taz pathway. Am J Physiol Lung Cell Mol Physiol 321:L814-L826 (2021). PubMed: 34431413
- Kinoshita A et al. Itpr1 regulates the formation of anterior eye segment tissues derived from neural crest cells. Development 148:N/A (2021). PubMed: 34338282
- Nguyen TM et al. The proportion of alveolar type 1 cells decreases in murine hypoplastic congenital diaphragmatic hernia lungs. PLoS One 14:e0214793 (2019). PubMed: 30995255
- Lang JF et al. Standard screening methods underreport AAV-mediated transduction and gene editing. Nat Commun 10:3415 (2019). PubMed: 31363095
- Inoue T et al. Upregulation of sphingosine-1-phosphate receptor 3 on fibroblast-like synoviocytes is associated with the development of collagen-induced arthritis via increased interleukin-6 production. PLoS One 14:e0218090 (2019). PubMed: 31173610