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Immunology Immunoglobulins Heavy Chain IgG

山羊抗大鼠IgG H&L (HRP)预吸附二抗(ab7097)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (37)

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Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)
  • IHC - Wholemount - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)

Key features and details

  • Goat Anti-Rat IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot blot

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概述

  • 产品名称

    山羊抗大鼠IgG H&L (HRP)预吸附二抗
    参阅全部 IgG 二抗
  • 宿主

    Goat
  • 靶标种属

    Rat
  • 经测试应用

    适用于: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot blotmore details
  • 最小交叉反应


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • 免疫原

    Full length native Rat IgG (purified).

  • 偶联物

    HRP

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明

    Horseradish Peroxidase (HRP)
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rat
    • IgG
    • Enzyme
    • HRP

相关产品

  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7097于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/500 - 1/1500.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
ELISA
1/50000.
Dot blot
Use at an assay dependent dilution.
说明
ICC/IF
1/500 - 1/1500.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
ELISA
1/50000.
Dot blot
Use at an assay dependent dilution.

图片

  • Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)
    Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)
    Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097) at 1/1000 dilution + Rat IgG, 50 ng


    Both the heavy and light chains of rat IgG are detected.

  • IHC - Wholemount - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)
    IHC - Wholemount - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)This picture was kindly supplied as part of the review submitted by Helen Makarenkova.

    Whole mount immunostaining of E11.5 mouse embryo (Blood vessels in the embryonic head). This staining was performed with rat monoclonal antibody to Pecam followed by staining with ab7097 (HRP conjugated).

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (37)

发表研究结果有使用 ab7097?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7097 被引用在 37 文献中.

  • Liu H  et al. Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis. Oxid Med Cell Longev 2022:8011850 (2022). PubMed: 35419170
  • Ou Y  et al. Targeting Antisense lncRNA PRKAG2-AS1, as a Therapeutic Target, Suppresses Malignant Behaviors of Hepatocellular Carcinoma Cells. Front Med (Lausanne) 8:649279 (2021). PubMed: 33928106
  • Xie B  et al. lncRNA MEG3 Downregulation Relieves Intracerebral Hemorrhage by Inhibiting Oxidative Stress and Inflammation in an miR-181b-Dependent Manner. Med Sci Monit 27:e929435 (2021). PubMed: 34267173
  • Tong C  et al. Tandem Mass Tag-Based Quantitative Proteomic Analysis Reveals Pathways Involved in Brain Injury Induced by Chest Exposure to Shock Waves. Front Mol Neurosci 14:688050 (2021). PubMed: 34630032
  • Liu G  et al. CDK6 is stimulated by hyperthermia and protects gastric cancer cells from hyperthermia-induced damage. Mol Med Rep 23:N/A (2021). PubMed: 33537819
View all Publications for this product

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Please see customers inquiry below:

I bought Primary and secondary antibodies from sapphire biosciences almost two years ago. Upon arrival I immediately aliqoute into smaller volume and kept them in -80 degree ever since. Do you think they are still okay to use?

Primary Ab: F4/80 monoclonal antibody; Cat#AB6640 (Abcam)

Secondary Ab: Goat polyclonal secondary antibody to Rat IgG-H &L (HRP), pre-adsorbed; Cat#AB7097 (Abcam)




Customer Service & Technical Support | mailto:julic@sapphirebioscience.com

126 Cope Street │ Waterloo NSW 2017 │ Australia

Ph: 1800 062 088│ Fax: +61 2 9698 1022 | http://www.sapphirebioscience.com/

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Abcam community

Verified customer

Asked on Jan 17 2013

Answer

Thank you for contacting us.

Please reffer the customer to our FAQs and our Storage guide:

"For many of our antibodies, freezing at -20oC or -80oC in small aliquots is the optimal storage condition. Aliquotting minimizes damage due to freezing and thawing, as well as contamination introduced by pipetting from a single vial multiple times. Aliquots are to be frozen and thawed once, with any remainder kept at 4oC. Upon receiving the antibody, centrifuge at 10,000 x g for 20 seconds to pull down solution that is trapped in the threads of the vial, and transfer aliquots into low-protein-binding microcentrifuge tubes. The size of the aliquots will depend on how much one typically uses in an experiment. Aliquots should be no smaller than 10 µl; the smaller the aliquot, the more the stock concentration is affected by evaporation and adsorption of the antibody onto the surface of the storage vial.


Enzyme-conjugated antibodies, should not be frozen at all and should instead be kept at 4oC. Freezing and thawing will reduce enzymatic activity in addition to affecting the antibody binding capacity.

However, when the HRP conjugated abs wasn't thought in between, the perfomance should still be ok.

The rule of the thump is that a -80C stored antibody should be fine for the next 10 years.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

回复于 Jan 17 2013

Question

We received a recommendation for a secondary antibody that is inappropriate for our experiment (cross-reactive with mouse IgG). Please send a secondary that is cross-absorbed to remove reactivity with mouse IgG.

Read More

Abcam community

Verified customer

Asked on Sep 16 2011

Answer

As requested, I have issued a free of charge replacement for ab7097, anti-rat IgG cross-absorbed to remove reactivity with mouse IgG. I apologize for the misunderstanding on our part and wish you the best of luck with your research.

Read More

Abcam Scientific Support

回复于 Sep 16 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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