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山羊抗大鼠IgG H&L (HRP polymer) (ab214882)

Reviews (3) Submit a question References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)

    Key features and details

    • Goat Anti-Rat IgG H&L (HRP polymer)
    • Conjugation: HRP polymer
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: Immunoelectrophoresis, IHC-P

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 6nm HRP PE PE Texas Red ® TRITC Unconjugated Unconjugated

    概述

    • 产品名称

      山羊抗大鼠IgG H&L (HRP polymer)
      参阅全部 IgG 二抗

    • 宿主

      Goat

    • 靶标种属

      Rat

    • 特异性

      Based on IEP, ab214882 reacts with heavy (gamma) chains on rat IgG, light chains on all rat immunoglobulins. No cross-reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or mouse.

    • 经测试应用

      适用于: Immunoelectrophoresis, IHC-Pmore details

    • 免疫原

      Full length protein corresponding to Rat IgG. (Purified Rat IgG, whole molecule).

    • 偶联物

      HRP polymer

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      Preservative: 0.1% Proclin 300
      Constituents: 1% BSA, 98% PBS
    • Concentration information loading...

    • 纯度

      Affinity purified

    • 纯化说明

      ab214882 was affinity purified using solid phase Rat IgG. Purity >95% by SDS-PAGE.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab214882于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Immunoelectrophoresis
    Use at an assay dependent concentration.

    This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour.
    IHC-P (2)
    Use at an assay dependent concentration.
    说明
    Immunoelectrophoresis
    Use at an assay dependent concentration.

    This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour.
    IHC-P
    Use at an assay dependent concentration.

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (ab214882)

      C57BL/6 mouse spleen stained with 1 µg/ml of rat anti-mouse CD45R/B220 detected using ab214882 Goat Anti-Rat IgG H&L (HRP polymer).

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (3)

    发表研究结果有使用 ab214882？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab214882 被引用在 3 文献中.

    • Kireeva G  et al. Modeling of Chemoperfusion vs. Intravenous Administration of Cisplatin in Wistar Rats: Adsorption and Tissue Distribution. Molecules 25:N/A (2020). PubMed: 33076418
    • Li X  et al. Ubiquitination of RIPK1 regulates its activation mediated by TNFR1 and TLRs signaling in distinct manners. Nat Commun 11:6364 (2020). PubMed: 33311474
    • Nakashima H  et al. Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma. Clin Cancer Res 24:2574-2584 (2018). PubMed: 29511029

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    1-3 of 3 Abreviews or Q&A

    CD31 staining in FFPE mouse kidney

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    The FFPE mouse kidney sections were deparaffinized and rehydrated. For antigen retrieval, the tissue sections were boiled in Sodium Citrate pH6 in a pressure cooker for 10 minutes. The tissue sections were blocked with 2% BSA for an hour at room temperature. Incubated with rat anti-CD31 primary antibody (1:300) overnight at 4oC. After 3 washes with TBST, the sections were incubated with goat anti-rat HRP polymer secondary antibody (ab214882) for 90 minutes at room temperature. The sections were developed in DAB after 3 washes with TBST.
    The CD31 is very distinct in the mouse kidney section.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 02 2018

    Goat anti-rat IgG HRP-Polymer for IHC on frozen sections

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Frozen OCT-embedded mouse spleen:
    Section was air-dried
    Section fixed in aceton, -20C, for 10 minutes
    Peroxidase block Dako 10'
    Protein block Dako 10'
    Anti-mouse B220 (Biolegend, 0.5 mg/ml, 1:100) 30' RT
    Goat anti-rat IgG PolymerHRP 20' RT
    Colour developed with DAB
    Counterstained with haematoxylin and mounted
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Davide Lucchesi

    Verified customer

    提交于 Aug 10 2017

    Goat anti-rat IgG HRP-Polymer for IHC on paraffin sections

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Paraffin-embedded mouse spleen:
    Section was deparaffinized
    Antigen retrieval Dako, pH6 for 15' in microwave
    Peroxidase block Dako 10'
    protein block Dako 10'
    Anti-mouse B220 (Biolegend, 0.5 mg/ml, 1:100) 30' RT
    Goat anti-rat IgG PolymerHRP 20' RT
    Colour developed with DAB
    Counterstained with haematoxylin and mounted
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Davide Lucchesi

    Verified customer

    提交于 Aug 01 2017

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