山羊抗大鼠IgG H&L (HRP) (ab205720)
Key features and details
- Goat Anti-Rat IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, IP, ELISA, IHC-P
Related conjugates and formulations
概述
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产品名称
山羊抗大鼠IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rat -
特异性
The antibody used for conjugation reacts with rat immunoglobulins of all classes. Cross-reactions as determined by ELISA for the unconjugated antibody (ab182018): Chicken IgY, rabbit IgG and human IgG, less than 2%. Mouse IgG, less than 7%. -
经测试应用
适用于: WB, IP, ELISA, IHC-Pmore details -
免疫原
The details of the immunogen for this antibody are not available.
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab205720于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/2000 - 1/20000.
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
1/1000 - 1/10000.
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说明 |
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WB
1/2000 - 1/20000. |
IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-P
1/1000 - 1/10000. |
图片
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All lanes : Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1 µg/ml
Lane 1 : Liver (Human) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6161 overnight at 4°C. Antibody binding was detected using ab205720, and visualised using ECL development solution ab133406.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP) (ab205720)
IHC image of tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab6160 at 2ug/ml dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature.
An HRP-conjugated secondary (Ab205720, 1/2000 dilution) was used for 1hr at room temperature.
The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : No Primary Antibody
Lane 1 : Liver (Human) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/2000 dilution
Performed under reducing conditions.
Exposure time: 15 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was incubated overnight with 2% Bovine Serum Albumin at 4°C. Any non-specific background binding was assessed by incubating the membrane with only the secondary antibody (ab205720), and visualised using ECL development solution ab133406.
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All lanes : Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1 µg/ml
Lane 1 : Liver (Human) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : ab205720 (Left Image) at 1/5000 and a competitor secondary (Right Image) at 1/5000. Notice the increased background of the competitor product.
Performed under reducing conditions.
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6161 overnight at 4°C. Antibody binding was detected using ab205720 (Left Image) and a competitor secondary (Right Image), and visualised using ECL development solution ab133406.
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All lanes : No Primary Antibody
Lane 1 : Liver (Human) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : ab205720 (Left Image) 1/2000 and a competitor secondary (Right Image) 1/2000. Notice the increased background of the competitor product.
Performed under reducing conditions.
Exposure time: 15 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was incubated overnight with 2% Bovine Serum Albumin at 4°C. Any non-specific background binding was assessed by incubating the membrane with ab205720 (Left Image) and a competitor secondary (Right Image), and visualised using ECL development solution ab133406.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab205720 被引用在 11 文献中.
- Saddawi-Konefka R et al. Lymphatic-preserving treatment sequencing with immune checkpoint inhibition unleashes cDC1-dependent antitumor immunity in HNSCC. Nat Commun 13:4298 (2022). PubMed: 35879302
- Fallatah W et al. A Pex7 Deficient Mouse Series Correlates Biochemical and Neurobehavioral Markers to Genotype Severity-Implications for the Disease Spectrum of Rhizomelic Chondrodysplasia Punctata Type 1. Front Cell Dev Biol 10:886316 (2022). PubMed: 35898397
- Lin H et al. Nerve growth factor regulates liver cancer cell polarity and motility. Mol Med Rep 23:N/A (2021). PubMed: 33649819
- Stewart TA et al. Mammary mechanobiology - investigating roles for mechanically activated ion channels in lactation and involution. J Cell Sci 134:N/A (2021). PubMed: 33262312
- Vu LD et al. Deficiency in ST2 signaling ameliorates RSV-associated pulmonary hypertension. Am J Physiol Heart Circ Physiol 321:H309-H317 (2021). PubMed: 34170196