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山羊抗大鼠IgG H&L (FITC) (ab6840)

Submit a review Q&A (1)References (37)
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (FITC) (ab6840)

    Key features and details

    • Goat Anti-Rat IgG H&L (FITC)
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 Gold 10nm HRP HRP polymer PE PE Texas Red ® TRITC Unconjugated Unconjugated

    概述

    • 产品名称

      山羊抗大鼠IgG H&L (FITC)
      参阅全部 IgG 二抗

    • 宿主

      Goat

    • 靶标种属

      Rat

    • 经测试应用

      适用于: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

    • 免疫原

      Rat whole molecule

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      pH: 6.50
      Preservative: 0.01% Sodium azide
      Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate
    • Concentration information loading...

    • 纯度

      Affinity purified

    • 纯化说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • 共轭说明

      Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6840于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.
    说明
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.

    图片

    • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (FITC) (ab6840)
      Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (FITC) (ab6840)
      Ab6840 was used at dilution 1/500 with the primary antibody ab6326 in ICC/IF. See the review on ab6326.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (37)

    发表研究结果有使用 ab6840？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6840 被引用在 37 文献中.

    • Amirrad F  et al. Hypertrophic and fibrotic human PKD hearts are associated with macrophage infiltration and abnormal TGF-β1 signaling. Cell Tissue Res 391:189-203 (2023). PubMed: 36376769
    • Xiao J  et al. IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties. Cell Mol Biol Lett 28:15 (2023). PubMed: 36849929
    • Liang C  et al. Neuroprotection by Nrf2 via modulating microglial phenotype and phagocytosis after intracerebral hemorrhage. Heliyon 9:e13777 (2023). PubMed: 36852060
    • Liu H  et al. Crocin improves the renal autophagy in rat experimental membranous nephropathy via regulating the SIRT1/Nrf2/HO-1 signaling pathway. Ren Fail 45:2253924 (2023). PubMed: 37724538
    • Ou S  et al. Tibial cortex transverse transport potentiates diabetic wound healing via activation of SDF-1/CXCR4 signaling. PeerJ 11:e15894 (2023). PubMed: 37727693
    View all Publications for this product

    客户评价及客户问答

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    Question

    Hi, Do you have any secondary antibody (IHC-Fr) that works good for ab6640 Rat F4/80? The fluorescent conjugate could be either CY2 or FITC. Thank you. Best,

    Read More
    Answer

    Thank you for contacting us. The anti Rat secondary antibody compatible for ab6640 is CY2 conjugate: ab6952; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6952.html FITC conjugate: ab6840; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6840.html ab7093: https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab7093.html There are many other different types of secondary antibodies available in our catalogue. Please try our secondary search tool on Abcam website. You can nicely select conjugate, format and species of required ab from drop down menu. Click the link to view the search tool: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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