为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    按产品类型
    蛋白质与肽
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Immunology Immunoglobulins Heavy Chain IgG

山羊抗大鼠IgG H&L (Cy5 ®)预吸附二抗(ab6565)

  • Datasheet
  • SDS
Submit a review Q&A (6)References (36)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed (ab6565)

    Key features and details

    • Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed
    • Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC, Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Fr

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm HRP HRP polymer PE PE Texas Red ® TRITC Unconjugated Unconjugated

    你可能会感兴趣

    一抗
    Product image
    Anti-SNAIL + SLUG antibody (ab167609)
    色原
    NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (ab7468)
    封闭
    Product image
    Normal Goat Serum (ab7481)

    查看更多相关产品

    概述

    • 产品名称

      山羊抗大鼠IgG H&L (Cy5 ®)预吸附二抗
      参阅全部 IgG 二抗
    • 宿主

      Goat
    • 靶标种属

      Rat
    • 经测试应用

      适用于: ICC, Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Frmore details
    • 最小交叉反应


      Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep
      To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
      more details
    • 免疫原

      Rat IgG, whole molecule (H&L)

    • 偶联物

      Cy5 ®. Ex: 650nm, Em: 667nm

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C.
    • 存储溶液

      pH: 6.5
      Preservative: 0.01% Sodium azide
      Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    • 共轭说明

      Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG
    • 克隆

      多克隆
    • 同种型

      IgG
    • 常规说明

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


      This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

    • 研究领域

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Rat
      • IgG
      • Fluorophore
      • Cy5

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6565于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF
    1/1000 - 1/5000.
    ELISA
    1/10000 - 1/50000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    说明
    ICC
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF
    1/1000 - 1/5000.
    ELISA
    1/10000 - 1/50000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.

    图片

    • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed (ab6565)
      Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed (ab6565)

      ab6565 was used at dilution 1/500 with the primary antibody ab6161 in ICC/IF. See the review on ab6161.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (36)

    发表研究结果有使用 ab6565?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6565 被引用在 36 文献中.

    • Tirado-Class N  et al. DCAF14 regulates CDT2 to promote SET8-dependent replication fork protection. Life Sci Alliance 7:N/A (2024). PubMed: 37940188
    • Bai M  et al. Substrate stiffness promotes dentinogenesis via LAMB1-FAK-MEK1/2 signaling axis. Oral Dis 30:562-574 (2024). PubMed: 36519511
    • Thakur BL  et al. SIRT1 Prevents R-Loops during Chronological Aging by Modulating DNA Replication at rDNA Loci. Cells 12:N/A (2023). PubMed: 37998365
    • Saha LK  et al. Replication-associated formation and repair of human topoisomerase IIIα cleavage complexes. Nat Commun 14:1925 (2023). PubMed: 37024461
    • Chang YC  et al. RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication. Open Biol 13:230068 (2023). PubMed: 37607592
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Question

     Dear technical support: This customer has highly interested in ab6565 Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5 ®), pre-adsorbed) and ab6346 (Anti-Tenascin C antibody [MTn-12]), this customer would like to use these antibodies to conduct ELISA assay. Therefore, he wants to ask for your help to offer him any suggestion (e.g. blocking buffer and washing Buffer and protocol etc…), would you please help this customer to solve the problem? Thanks for your kindly assistance. Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Oct 31 2011

    Answer

    Thank you very much for your interest in our products. I can confirm that ab6346 is tested and guaranteed for ELISA whereas ab6565 is not yet tested. Please find a testing discount offer below for ab6565. I attached protocols for indirect and direct ELISA for the information of your customer. These protocols and more can also be found using this link: https://www.abcam.com/index.html?pageconfig=popular_protocols   I hope this information is helpful. Please do not hesitate to contact me again with any further questions. If you do, could you please include more information about the planned experiments of your customer? This will make it easier for me to help your customer with more specific suggestions.   Testing Discount Offer: To our knowledge, ab6565 has not been tested in ELISA. Therefore, I can offer a discount off a future purchase if you buy ab6565 now, test it in ELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free secondary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab6565 in ELISA. I will then send a discount code. This code must be issued before purchasing ab6565 so please wait for my reply before ordering. 2. Purchase ab6565 either by phone, fax, or online (www.abcam.com). 3. Test it in ELISA. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any secondary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab6565 turns out to be unsuitable for ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

    Read More

    Abcam Scientific Support

    回复于 Oct 31 2011

    Question

    In the title of the attchament I said I used Cell cultures from bEnd3 cells. Yes, I though that the problem could be the secondary antibody but I bought it to abcam at the same time (the problem is the same) and to clarify this issue I need another primary of the same specie (rat) because I don't have it to test.   Thanks in advance,  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 19 2011

    Answer

    Thank you for your response and for clarifying the cell types used in the experiments.    I understand now that bEnd3 cells (immortalized mouse brain endothelial cell line) were stained for CD31. The protocol looks absolutely fine and it you have an alternative antibody which worked fine with these cells. I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.   I could raise either a credit note for the cost of  one vial of ab7388 and ab7388 since you have alternative antibodies; or send you two replacement vials (one of each)  free of charge. Please could you let me know how you wish to proceed with your enquiry? I look forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    回复于 Oct 19 2011

    Question

    I send in attach the protocol I used (that unswer to all questions of the inquiry). This protocol is optimized for a lot of antigens, namely CD31....   Fixative for 10 min at RT: PFA 4% Acetone PFA:Acetone (1:1) Wash 3x in PBS Permeabilize with Triton X-100 0,2%, 10 min, RT; Wash 3x in PBS Blockinh with BSA/PBS 1%, 40-60 min, RT; Wash in PBS Incubate 1st antibody, ON, 4ºC (rat CD31 abcam, ab7388) 1:50 1:100 1:500 1:1000 Wash in PBS Incubate with 2nd antibody in the dark, for 2h, RT (anti-rat abcam, ab6565) 1:1000 1:500 Wash in the dark Coverslip with Prolong Gold + DAPI. Fixative for 10 min at RT: PFA 4% Acetone PFA:Acetone (1:1) Wash 3x in PBS Permeabilize with Triton X-100 0,2%, 10 min, RT; Wash 3x in PBS Blockinh with BSA/PBS 1%, 40-60 min, RT; Wash in PBS Incubate 1st antibody, ON, 4ºC (rat CD31 abcam, ab7388) 1:50 1:100 1:500 1:1000 Wash in PBS Incubate with 2nd antibody in the dark, for 2h, RT (anti-rat abcam, ab6565) 1:1000 1:500 Wash in the dark Coverslip with Prolong Gold + DAPI.  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 19 2011

    Answer

    Thank you for your response. Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem. 1)  Sample: Could you specify the samples used for the staining i.e. species, cells (cultured) or tissues? 2) Detection system: Have you used the secondary antibody successfully with another primary antibody? Do you think that the problem may come from the secondary antibody? Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

    Read More

    Abcam Scientific Support

    回复于 Oct 19 2011

    Question

    Hi! For experiment design, I want to know if this antibody would cross react with mouse IgG? I couldn't fine the reactivity information of this Ab in your company's website. Is there any information about this? Very thanks for your help!!

    Read More

    Abcam community

    Verified customer

    Asked on Sep 28 2010

    Answer

    Thank you for your enquiry. I can confirm that ab6565 Goat polyclonal Secondary Antibody to Rat IgG has been cross adsorbed against mouse serum proteins and has less than 1% cross reactivity against mouse polyclonal IgG by ELISA testing. I hope this information will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    回复于 Sep 28 2010

    Question

    What is the concentration of this antibody? It is lot 168763.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 10 2006

    Answer

    Thank you for your enquiry. The concentration of this antibody is 1mg/ml. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Mar 10 2006

    Question

    I'm afraid I have some fairly basic questions, but I need a quick answer from a reliable source! I'm interested in buying a secondary antibody conjugated to a blue fluorochrome. Firstly, is Cy5.5 a blue fluorochrome as well as Cy5 and if so what is the difference? Also, am I right in thinking that Cy5 and AF 350 are the only blue fluorochromes that can be purchased conjugated to secondary antibodies? I was also wanting to check if these antibodies can be visualised through a DAPI filter on a normal fluorescence microscope and a confocal laser microscope. Providing I am barking up the right tree with these assumptions, I'm looking for an anti-mouse IgG and/or IgM antibody and I'm also considering an anti-rat IgG and an anti-rabbit IgG to use for triple immunolabelling on primary cell cultures (oligodendrocytes) and on paraffin pre-fixed and/or fresh frozen cryostats. Can you give me information on which of these antibodies you supply and particularly any that are good value or supplied in trial size volumes!

    Read More

    Abcam community

    Verified customer

    Asked on May 30 2004

    Answer

    Thanks for your enquiry,unfortunately we do not supply sample sized testers. As far as I am aware both CY5 and CY 5.5 are red. If you log on to our web page and click on secondary antibodies there is a list of all the secondary antibodies we supply along with a fluorophore data table.

    Read More

    Abcam Scientific Support

    回复于 Jun 02 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2025 艾博抗(上海)贸易有限公司版权所有