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山羊抗大鼠IgG H&L (Cy3 ®)预吸附二抗(ab98416)

Reviews (1) Submit a question References (4)

Key features and details

  • Goat Anti-Rat IgG H&L (Cy3 ®) preadsorbed
  • Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, Flow Cyt

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 6nm HRP HRP polymer PE PE Texas Red ® TRITC Unconjugated Unconjugated

概述

  • 产品名称

    山羊抗大鼠IgG H&L (Cy3 ®)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Rat

  • 经测试应用

    适用于: IHC-P, ICC/IF, Flow Cytmore details

  • 最小交叉反应


    Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details

  • 偶联物

    Cy3 ®. Ex: 552nm, Em: 565nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab98416于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/50 - 1/500.
ICC/IF
1/50 - 1/500.
Flow Cyt
1/100 - 1/1000.
说明
IHC-P
1/50 - 1/500.
ICC/IF
1/50 - 1/500.
Flow Cyt
1/100 - 1/1000.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (4)

发表研究结果有使用 ab98416？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab98416 被引用在 4 文献中.

  • Liu X  et al. The AMPK-mTOR axis requires increased MALAT1 expression for promoting granulosa cell proliferation in endometriosis. Exp Ther Med 21:21 (2021). PubMed: 33235630
  • Gershman BW  et al. Tissue-specific Expression of Ribosomal Protein Paralogue eRpL22-like in Drosophila melanogaster Eye Development. Dev Dyn N/A:N/A (2020). PubMed: 32353187
  • Chauhan A  et al. The platelet receptor CLEC-2 blocks neutrophil mediated hepatic recovery in acetaminophen induced acute liver failure. Nat Commun 11:1939 (2020). PubMed: 32321925
  • Zietzer A  et al. The RNA-binding protein hnRNPU regulates the sorting of microRNA-30c-5p into large extracellular vesicles. J Extracell Vesicles 9:1786967 (2020). PubMed: 32944175

客户评价及客户问答

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CD3/CD19 positive splenocytes

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Goat polyclonal antibody to Rat IgG- H&L (Cy3®), ab98416, Lot: GR116866-2
Combined with anti-CD3 antibody (ab16669) and anti-CD19 antibodies (ab25232) and Goat anti-Rabbit IgG H&L (DyLight® 488) secondary antibody ab96895:

Following fixation in in 4% paramformaldehyde in PBS for 15 min, slides were blocked with 10% goat serum for 30 min and washed 3 times with ice-cold PBS, and incubated with mixture of anti-CD3 (ab16669) and anti-CD19 antibodies (ab25232), diluted 1:200 in PBST (1× PBS with 0.05% Tween-20), 1% BSA for 1 hour in a humidified chamber at room temperature.
Slides were washed 4 times in PBS (5 min each) and incubated for 1 hour in dark at room temperature with mixture of secondary antibodies: Goat DyLight® 488-conjugated anti-Rabbit (ab96895) and Goat Cy3®-conjugated antibody to Rat IgG - H&L (ab98416), diluted 1:200 in PBST, 1% BSA.
Slides were washed 3 times with PBS in dark (each wash for 5 min) and counterstained with 0.1 µg/ml DAPI for 5 min and finally rinsed with PBS, left 10 minutes for air-drying in dark and finally mounted.
CD3 antigen appears green CD19 red and the nuclei are stained blue.
Fluorescent microscope pictures at 10x.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Nasr Hemdan

Verified customer

提交于 Jul 15 2013

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