Goat Anti-Rat IgG H&L (FITC)

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (FITC) (AB6840)

ab6840 was used at dilution 1/500 with the primary antibody ab6326 in ICC/IF. See the review on ab6326.

• WB

Lab

Western blot - Goat Anti-Rat IgG H&L (FITC) (AB6840)

This data was developed using ab68460, the same antibody clone in a different buffer formulation.

Lanes 1 - 4 : Merged signal (red and green). Green - ab68460 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab68460 was shown to specifically react with KRT7 (Cytokeratin 7) in wild-type A549 cells as signal was lost in KRT7 knockout cells. Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. ab68460 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

KRT7 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (FITC) (ab6840) at 1/10000 dilution

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