AB6846

Goat Anti-Rat IgG H&L (Alkaline Phosphatase)

Name: 山羊抗大鼠IgG H&L (Alkaline Phosphatase) (ab6846)| Abcam中文官网
Brand: Abcam Limited
SKU: AB6846
Price: 2398 CNY
Availability: InStock

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(5 Publications)

Suitable for Dot, IHC-Fr, IHC-P, IM, ICC/IF, WB, ELISA. Cited in 5 publications.

查看别名

Ig gamma-1 chain C region

2 Images

Unknown

Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (AB6846)

All lanes:

Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (<a href='/products/primary-antibodies/tubulin-antibody-yl1-2-loading-control-ab6160'>ab6160</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/5000 dilution

Lanes 3 - 4:

Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/20000 dilution

Lanes 5 - 6:

Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/50000 dilution

Predicted band size: 36 kDa

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Supplier Data

Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (AB6846)

Western blot analysis of ab6846 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Rat IgG at 50 ng

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6846) at 1/1000 dilution

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关键信息

宿主种属

Goat

靶标种属

Rat

靶标亚型

IgG

靶标特异性

Heavy & Light chains

最低交叉反应

预吸附

偶联物

Alkaline Phosphatase

激发波长/发射波长

应用

IHC-Fr, Dot, IHC-P, IM, ICC/IF, WB, ELISA

applications

克隆

Polyclonal

亚型

IgG

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/200 - 1/1000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/15000", "notes":"" } } }

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产品详情

Part of the AbExcel range.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

纯化说明

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

存储溶液

pH: 8 Preservative: 0.1% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride

运输条件

Blue Ice

产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information Ig gamma-1 chain C region

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

BMC microbiology 24:96 PubMed38521930

2024

Human-derived bacterial strains mitigate colitis via modulating gut microbiota and repairing intestinal barrier function in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Juanjuan Dai,Mingjie Jiang,Xiaoxin Wang,Tao Lang,Leilei Wan,Jingjing Wang

Journal of lipid research 64:100450 PubMed37751791

2023

Sphingosine kinase 1 is involved in triglyceride breakdown by maintaining lysosomal integrity in brown adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Ichi Morishige,Kazuaki Yoshioka,Hiroki Nakata,Kazuhiro Ishimaru,Naoto Nagata,Tamotsu Tanaka,Yoh Takuwa,Hitoshi Ando

PloS one 17:e0264572 PubMed35271602

2022

Topical estrogen application promotes cutaneous wound healing in db/db female mice with type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Kanae Mukai,Shin-Ichi Horike,Makiko Meguro-Horike,Yukari Nakajima,Arya Iswara,Toshio Nakatani

PloS one 13:e0198531 PubMed29902192

2018

Presentation matters: Impact of association of amphiphilic LPS with serum carrier proteins on innate immune signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Loreen R Stromberg,Heather M Mendez,Jessica Z Kubicek-Sutherland,Steven W Graves,Nicolas W Hengartner,Harshini Mukundan

Insects 3:1190-9 PubMed26466734

2012

Myosin Gene Expression and Protein Abundance in Different Castes of the Formosan Subterranean Termite (Coptotermes formosanus).

Applications

Unspecified application

Species

Unspecified reactive species

Matthew R Tarver,Christopher B Florane,Christopher P Mattison,Beth A Holloway,Alan Lax

View all publications

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Goat Anti-Rat IgG H&L (Alkaline Phosphatase)