山羊抗大鼠IgG Fc (DyLight® 550)预吸附二抗(ab96972)
Key features and details
- Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
概述
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产品名称
山羊抗大鼠IgG Fc (DyLight® 550)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rat -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Reduced cross-reactivity to human and mouse IgG was detected.
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经测试应用
适用于: IHC-P, ICC/IF, Flow Cytmore details -
最小交叉反应
Human, Mouse more details -
偶联物
DyLight® 550. Ex: 562nm, Em: 576nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550. -
克隆
多克隆 -
同种型
IgG -
常规说明
DyLight® 550 replaces DyLight® 549. -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96972于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/500.
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| ICC/IF | (1) |
1/50 - 1/500.
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| Flow Cyt |
1/50 - 1/200.
|
| 说明 |
|---|
|
IHC-P
1/50 - 1/500. |
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ICC/IF
1/50 - 1/500. |
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Flow Cyt
1/50 - 1/200. |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.Anti dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 550), pre-adsorbed (ab96972) was used as a secondary antibody at a concentration of 1/500.
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)This image is courtesy of an Abreview submitted by Ya ZhouImmunocytochemsitry/Immunofluorescence analysis of HEK293 cells using Goat Anti-Rat IgG Fc (DyLight® 550) preabsorbed (ab96972) in yellow labelling a cell surface protein. Cells were incubated with the secondary antibody (1/500 in PBS + 2% BSA) for 1 hour at room temperature.
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Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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