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山羊抗兔IgG H&L (TRITC)预吸附二抗(ab7087)

Submit a review Q&A (2)References (3)

Key features and details

  • Goat Anti-Rabbit IgG H&L (TRITC) preadsorbed
  • Conjugation: TRITC. Ex: 547nm, Em: 572nm
  • Host species: Goat
  • Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA

概述

  • 产品名称

    山羊抗兔IgG H&L (TRITC)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Rabbit

  • 特异性

    No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

  • 经测试应用

    适用于: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details

  • 最小交叉反应


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • 免疫原

    Full length native Rabbit IgG (purified).

  • 偶联物

    TRITC. Ex: 547nm, Em: 572nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7087于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr
ELISA
说明
应用说明
Flow Cyt: Use at an assay dependant concentration.
IHC-P: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
IM: Use at an assay dependant concentration.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (3)

发表研究结果有使用 ab7087？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7087 被引用在 3 文献中.

  • Xu L  et al. MEF/KSF-conditioned culture medium: An effective method for in vitro culture of mouse dermal papilla cells with osteogenic differentiation potential. Exp Ther Med 22:828 (2021). PubMed: 34149874
  • Zhou Y  et al. Anti-CD206 antibody-conjugated Fe3O4-based PLGA nanoparticles selectively promote tumor-associated macrophages to polarize to the pro-inflammatory subtype. Oncol Lett 20:298 (2020). PubMed: 33101492
  • Liu Y  et al. PKCd promotes fertilization of mouse embryos in early development via the Cdc25B signaling pathway. Exp Ther Med 18:3281-3290 (2019). PubMed: 31602201

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Re: the tetra methyl rhodamine isocyanate conjugated to this product. Is the dye used the 5 isomer, 6 isomer, or mixed isomers? ie tetramethylrhodamine-5-isothiocyanate (5-TRITC G isomer), tetramethylrhodamine-6-isothiocyanate (6-TRITC R isomer),or tetramethylrhodamine-5-(and-6)-isothiocyanate (5(6)-TRITC) *mixed isomers*

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Answer

Thank you for your recent technical service inquiry. The TRITC used for our conjugations is tetramethlyrodamine-5-(and-6)-isothiocyanate (mixed isomers). Please feel free to contact me for additional assistance regarding this issue.

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Question

I would like to ask you the difference between ab27089 and ab4055. I'd also like to know whether your goat anti-rabbit IgG (Rhodamine-conjugated); ab7087 or ab6718, is working well as a secondary antibody for these anti-CD8 polyclonal as well as the difference between ab7087 and ab6718. Thanks in advance.

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Answer

Thank you for your enquiry. 1) Ab27089 is supplier as a pre diluted product and Ab4055 is not. 2) We do not have specific feedback in relation to certain secondary Abs functioning in conjunction with our antibodies; however a list of compatible secondary Abs are cited at the base of each online data sheet for you to choose from. I would expect any non rabbit species anti rabbit IgG with a suitable conjugation to function. 3)Looking at products Ab7087 and Ab6718 the only obvious difference that I am able to spot is the different concentrations of the product. I will pass this information onto my colleague sin business development since it is possible that we have added a duplicate product in error. Should you have any further questions then please do not hesitate to get back in contact with me.

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