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Immunology Immunoglobulins Heavy Chain IgG

山羊抗兔IgG H&L (HRP) (ab97051)

  • Datasheet
  • SDS
Reviews (10)Q&A (28)References (696)

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Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Key features and details

  • Goat Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

Conjugates logo Related conjugates and formulations

Carrier Free Carrier Free Carrier Free Carrier Free Carrier Free Carrier Free Carrier Free Unconjugated Unconjugated Unconjugated Unconjugated Unconjugated Unconjugated Unconjugated

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概述

  • 产品名称

    山羊抗兔IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 宿主

    Goat
  • 靶标种属

    Rabbit
  • 特异性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • 经测试应用

    适用于: ICC, IHC-P, ELISA, WBmore details
  • 偶联物

    HRP

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 6.8
    Constituents: 0.2% BSA, PBS, 0.05% CMIT/MIT based preservative
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 共轭说明

    Molar enzyme/ antibody protein ratio is 4:1
  • 克隆

    多克隆
  • 同种型

    IgG
  • 常规说明

    Part of the AbExcel range.
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Enzyme
    • HRP

相关产品

  • Alternative Versions

    • Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)
  • Related Products

    • Normal Goat Serum (ab7481)
  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab97051于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use at an assay dependent concentration.
IHC-P
1/200 - 1/5000.
ELISA
1/10000 - 1/100000.
WB (10)
1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000
说明
ICC
Use at an assay dependent concentration.
IHC-P
1/200 - 1/5000.
ELISA
1/10000 - 1/100000.
WB
1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

图片

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    All lanes : Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063) at 1/1000 dilution

    Lane 1 : Rat pituitary whole tissue lysate
    Lane 2 : Mouse pituitary whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution


    Exposure time: 1st lane: 85 seconds
    2nd lane: 32 seconds

    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    All lanes : Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063) at 1/1000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell). Whole cell lysates
    Lane 2 : T-47D (human mammary gland ductal carcinoma epithelial cell). Whole cell lysates
    Lane 3 : MDA-MB231 (Human breast adenocarcinoma epithelial cell) Whole cell lysates (Negative control)
    Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) Whole cell lysates (Negative control)
    Lane 5 : Human uterus whole tissue lysate
    Lane 6 : Human ovary whole tissue lysate
    Lane 7 : Human ovary cancer whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Exposure time: 50 seconds


    Blocking and diluting buffer: 5% NFDM/TBST. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    Immunohistochemical staining of paraffin embedded human endometrial carcinoma with purified ab32063 at a working dilution of 1 in 200. The secondary antibody used is ab97051, a HRP goat anti-rabbit IgG (H+L), at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)Mamedov et al PLoS One. 2017 Aug 21;12(8):e0183589. doi: 10.1371/journal.pone.0183589. eCollection 2017. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Western blot analysis of co-expression Bacillus anthracis PA83 (A), Pfs48/45 (B) and Pfs48/45-10C with bacterial Endo H or PNGase F in N. benthamiana plants.

    (A) Western blot analysis of co-expression of PA83. Lanes: 1- N. benthamiana plant was infiltrated with pBI-PA83 construct, for the production of glycosylated PA83, 2,3- N. benthamiana plants were infiltrated with combinations of the pBI-Endo H/pBI-PA83 or pBI-PNGase F/pBI-PA83 constructs, for the production of Endo H (2) or PNGase F (3) deglycosylated PA83 proteins.

    (B) Western blot analysis of co-expression of Pfs48/45. Lanes: 1-N. benthamiana plant was infiltrated with pEAQ-Pfs48/45 construct for the production of glycosylated Pfs48/45;2,3- N. benthamiana plants were infiltrated with combinations of the pBI-Endo H/pEAQ-Pfs48/45 or pBI-PNGase F/pEAQ-Pfs48/45constructs for the production of Endo H (2) and PNGase F (3) deglycosylated Pfs48/45 proteins.

    (C) Western blot analysis of co-expression of Pfs48/45-10C. Lanes: 1- N. benthamiana plant was infiltrated with pEAQ-Pfs48/45-10C construct for the production of glycosylated Pfs48/45-10C; 2,3- N. benthamiana plants were infiltrated with combinations of the pBI-Endo H/pEAQ-Pfs48/45 or pBI-PNGase F/pEAQ-Pfs48/45constructs for the production of Endo H (2) and PNGase F (3) deglycosylated Pfs48/45-10C proteins. gPA83- glycosylated PA83; dPA83- deglycosylated PA83; gPfs48/45: glycosylated Pfs48/45; dPfs48/45: deglycosylated Pfs48/45; gPfs48/45-10C: glycosylated Pfs48/45-10C; dPfs48/45-10C: deglycosylated Pfs48/45-10C.

    M: MagicMark XP Western Protein Standard. PA83 proteins were detected using the anti-Bacillus anthracis protective antigen antibody BAP0101 (Cat. No. ab1988, Abcam); Ps48/45, Endo H or PNGase F proteins were detected using the anti-FLAG antibody. Pfs48/45-10C protein was detected using the purified anti-His Tag antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30mins. The section was incubated with ab8227, 3 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97051, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)This image is courtesy of an anonymous Abreview.
    Anti-LAMP2 antibody (ab37024) at 1/1000 dilution + Mouse brain whole tissue lysate at 30 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 1 minute


    10 % gel. Blocked with 5% BSA for 2 hours at 25°C.

    Incubated with the primary antibody for 1 hour in TBS-tween at 25°C.

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    All lanes : Anti-beta Actin antibody (ab8227) at 1 µg/ml

    All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Rabbit polyclonal to GNAT2 (ab97501) at 1/2000 dilution
    Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Lanes 5-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (696)

发表研究结果有使用 ab97051?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab97051 被引用在 696 文献中.

  • Zhang W  et al. Dexmedetomidine inhibits the growth and metastasis of esophageal cancer cells by down-regulation of lncRNA MALAT1. Kaohsiung J Med Sci 38:585-593 (2022). WB ; Human . PubMed: 35199933
  • Liu YK  et al. Low expression of FXYD5 reverses the cisplatin resistance of epithelial ovarian cancer cells. Histol Histopathol N/A:18310 (2021). PubMed: 33570156
  • Helmer RA  et al. Helicase-like transcription factor-deletion from the tumor microenvironment in a cell line-derived xenograft model of colorectal cancer reprogrammed the human transcriptome-S-nitroso-proteome to promote inflammation and redirect metastasis. PLoS One 16:e0251132 (2021). PubMed: 34010296
  • Jimenez-García MP  et al. Regulation of sarcomagenesis by the empty spiracles homeobox genes EMX1 and EMX2. Cell Death Dis 12:515 (2021). PubMed: 34016958
  • Liu W  et al. Long non-coding RNA LINC01215 promotes epithelial-mesenchymal transition and lymph node metastasis in epithelial ovarian cancer through RUNX3 promoter methylation. Transl Oncol 14:101135 (2021). PubMed: 34052627
View all Publications for this product

客户评价及客户问答

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提交评价 提交问题

1-10 of 38 Abreviews or Q&A

Worked well with Anti-IKB alpha antibody [EP697] (ab76429)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Goat Anti-Rabbit IgG H&L (HRP) ab97051 worked well with Anti-IKB alpha antibody [EP697] (ab76429) in Western Blot.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 24 2020

A good 2nd antibody for WB

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
This is a good 2nd antibody for WB assay.
Sample: 50 µg HeLa whole cell lysates.
Primary antibody: anti-human CCT7 rabbit polyclonal antibody from ThermoFisher Scientific (Cat# A304-730A-M).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 24 2020

second goat anti-rabbit antibody with HRP

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
This antibody works well for western blotting assay. The antigen I detected in the attached blot is 42 kD, the result looks great with this antibody (1:5,000 dilution).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 27 2018

works stably at a dilution of 1:4,000

Good
Abreviews
Abreviews
abreview image
Application
Western blot
this antibody works stably at a dilution of 1:4,000 for western blot assay.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 08 2016

good 2nd Ab for WB

Excellent
Abreviews
Abreviews
Application
Western blot
always works for WB at a dilution of 1:5,000.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 02 2015

Great secondary, works as promised.

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
We stock and use this for many of our westerns, gets the job done!
("B" and "G" are brain and gonad samples.)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Sebastian Alvarado

Verified customer

提交于 Aug 21 2015

Western Blot on Neuro2A cells

Good
Abreviews
Abreviews
abreview image
Application
Western blot
1. The concentration of Goat Anti-Rabbit IgG H&L (HRP) used for this experiment is 1:5000 and incubated for 2 hours.
2. Proteins were blocked with 5% BSA in TBS with 0.1% TritonX-100.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Lenin Veeraval

Verified customer

提交于 Jun 18 2015

excellent anti rabbit secondary antibody for WB

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Abcam's anti-rabbit HRP is very reliable and economical to use. We ended up using 10x less than our old antibody from a different company. Since it is so robust, we started calling it the "epic rabbit antibody" to distinguish it from the old one we have in the lab. The lower band in the gel corresponds to beta-tubulin (ab6046) followed by anti-rabbit HRP (ab97051) while the upper band is a different 70kDa protein followed by anti-mouse HRP (ab97023).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 21 2014

Question

Estimada
El anticuerpo a funcionado perfectamente, por lo que puedes mandar la segunda unidad cuando quieras.
Muchas gracias por todo

Read More

Abcam community

Verified customer

Asked on May 29 2012

Answer

Gracias por tu respuesta. Me alegra oír que este secundario os ha dado buenos resultados.

Ya he realizado por tanto el nuevo pedido completamente gratuito del mismo anticuerpo: ab97051.

Recibiréis una confirmación automática del mismo con más detalles.

Si necesitáis cualquier información referente al pedido, no dudéis en contactarnos, referenciando el número de éste: XXXXX.

Si tuvierais cualquier otro problema, o requirierais mas información / asistencia, por favor, no dudéis en volvernos a contactar.

Read More

Abcam Scientific Support

回复于 May 29 2012

Question

Regarding your answer; i would like to add this comments

1/Lysis buffer. To extract the protein from my tissue; i have used Qproteome kit from Qiagen, protocol for tissue, with protease inhibitors and benzonase nuclease;

2/ Loading amount:
Try to load at least 25-30 ug total tissue lysate per lane onto the gel. I have already load 40ug of total protein. Do you means that i need to load less?

Regards

Read More

Abcam community

Verified customer

Asked on Mar 23 2012

Answer

Thank you for passing some information to me.
I understand that ab3583 has been used previously at 1:4000.
Have you had a chance to test this antibody at dilutions of 1/500 or 1/1000 (for 1 hr at room temperature or overnight at 4oC?
Would you prefer to get a new vial of the primary antibody? Please do let me know how you wish to proceed.
I look forward to hearing from you soon.

Read More

Abcam Scientific Support

回复于 Mar 23 2012

1-10 of 38 Abreviews or Q&A

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