山羊抗兔IgG H&L (HRP) (ab6721)

Reviews (12)Q&A (14)References (4973)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
  • Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

Key features and details

  • Goat Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Fr

概述

  • 产品名称

    山羊抗兔IgG H&L (HRP)
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Rabbit

  • 经测试应用

    适用于: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Frmore details

  • 免疫原

    Rabbit IgG, whole molecule

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. Store In the Dark.

  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.

  • 共轭说明

    Horseradish Peroxidase (HRP)

  • 克隆

    多克隆

  • 同种型

    IgG

  • 常规说明

    HRP conjugated anti-rabbit secondary antibody optimized for western blot and immunohistochemistry. Some customers reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.

    Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

     

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6721于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (2)
1/1000.
WB (10)
1/2000 - 1/20000.

Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283).

Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances.
ELISA
1/120000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC
1/1000 - 1/5000.
IHC-Fr
1/1000.
说明
IHC-P
1/1000.
WB
1/2000 - 1/20000.

Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283).

Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances.
ELISA
1/120000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC
1/1000 - 1/5000.
IHC-Fr
1/1000.

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)Farah et al PLoS One. 2018 Feb 2;13(2):e0191526. doi: 10.1371/journal.pone.0191526. eCollection 2018. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Anti-phospho-tau immunostaining in Dryocopus lineatus.

    Tau-positivity in the midbrain (Panel A (shown) and B) and the corpus callosum (C) of the Dryocopus lineatus brain. The axonal tract staining demonstrates a thread-like pattern, similar to that seen with Gallyas sliver staining. Occasional intracellular tau-accumulations were identified within neurons (D).

    For full method, please see paper.

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)This image is courtesy of an Abreview submitted by Maryna Polyakova.
    All lanes : Anti-NEK7 antibody (ab80948) at 1/2000 dilution

    All lanes : Mouse brain tissue cytoplasmic lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds


    Blocked with 5% non-fat milk for 1 hour at 18°C

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)This image is courtesy of an anonymous Abreview.

    ab3580 staining glucocorticoid receptor in mouse epididymis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with Bouin's solution and blocked with 1.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000) for 14 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG H&L (ab6721) (1/200) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)Image is courtesy of an anonymous AbReview.

    Immunohistochemical analysis of PFA-fixed paraffin-embedded rat cardiac tissue sections, labeling Conexin 43 with ab117843 at a dilution of 1/500 incubated for 12 hours at 4°C in 1% BSA in TBS. Antigen retrival was via Tris-EDTA pH 9.0 (heat mediated). Blocking was 3% BSA incubated for 1 hour at 37°C. The secondary was ab6721 at 1/500.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

    ab6721 was used at dilution 1/100 with the primary antibody ab11370 in IHC-P. See the review on ab11370.

  • Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

    ab6721 was used at dilution 1/100 with the primary antibody ab35604 in IHC-Fr. See the review on ab35604.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (4973)

发表研究结果有使用 ab6721？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6721 被引用在 4973 文献中.

  • Park SH  et al. Glycogen Synthase Kinase-3 Isoform Variants and Their Inhibitory Phosphorylation in Human Testes and Spermatozoa. World J Mens Health 41:215-226 (2023). PubMed: 36047078
  • Pan J  et al. Exosomes derived from endothelial progenitor cells ameliorate glyoxylate deprivation (OGD)-induced neuronal apoptosis by delivering miR-221-3p. Histol Histopathol 38:423-430 (2023). PubMed: 36190183
  • Fang Q  et al. Embelin Enhances the Sensitivity of Renal Cancer Cells to Axitinib by Inhibiting HIF Signaling Pathway. Anticancer Agents Med Chem 23:807-816 (2023). PubMed: 36028958
  • Shen F  et al. Antihypertension effect of astragaloside IV during cerebral ischemia reperfusion in rats. Mol Med Rep 27:N/A (2023). PubMed: 36331022
  • Luo Y  et al. Metabolomic analysis and pharmacological validation of the cerebral protective effect of 3,4‑dihydroxybenzaldehyde on cerebral ischemia‑reperfusion injury. Mol Med Rep 27:N/A (2023). PubMed: 36367170
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 26 Abreviews or Q&A

Secondary antibody ab6721

 Excellent
Abreviews
abreview image
Application
Western blot
dilution buffer: 5% non-fat milk in PBS-Tween
dilution: 1/20000
incubation time: 1hour
exposure time: 30sec
Figure legend:
Western blot for HURP and alpha tubulin. MDA-MB-436 cells transfected with 50nM siRNA against HURP protein(lane 1) or 50nM non-targeting siRNA(lane 2) for 72hrs.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Christos Efstathiou

Verified customer

提交于 Mar 09 2022

Short incubation time needed

 Excellent
Abreviews
abreview image
Application
Western blot
A 45 minute incubation at 1/10,000 dilution in 5% skim milk in TBST is sufficient to obtain good signal
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Alina Radziwon

Verified customer

提交于 Jul 04 2022

Amazing WB Secondary

 Excellent
Abreviews
abreview image
Application
Western blot
reduced and denatured conditions
ab6721 incubated for 1hr at 20degrees in 5% milk at 1:5000
lanes 1 and 2 are human cell lysate (20ug/load) and the primary antibody was rabbit-anti-human
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 31 2022

Great signal

 Good
Abreviews
abreview image
Application
Western blot
Secondary antibody used for western blot. Dilution used: 1:5000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 20 2021

EXCELLENT SECONDARY ANTIBODY for WB

 Excellent
Abreviews
Application
Western blot
EXCELLENT SECONDARY ANTIBODY for WB
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Kyunga Kwak

Verified customer

提交于 May 07 2020

HRP for western blotting assay

 Good
Abreviews
Application
Western blot
I have been using this secondary antibody for my western blotting experiments and its working fine with all of proteins of our interest (i.e. Rodent CYP-450 enzymes like CYP-3A, 2D1, 2E1, 1A2, 2B1 etc.)
Before using this I had also explored the HRP antibody from other vendors but they were not so specific I was having lot of back ground noise on my membranes irrespective of varying dilutions and incubation times and so on.
So I would highly recommend using this for western blot experiments.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 20 2019

WB against mRFP (R) protein

 Excellent
Abreviews
abreview image
Application
Western blot
The antibody works very well, detects well two different protein tagged with mRFP.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 30 2019

Very sensitive and specific secondary antibody

 Excellent
Abreviews
abreview image
Application
Western blot
The dilution used for this secondary antibody was 1/20000 and we obtained intesnse specific bands even at very short incubation times. It works very well with phosphorylated proteins which sometimes are harder to detect (in these cases we use 1/10000 dilution or 1/5000).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 03 2018

ab6721

 Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
This HRP-conjugated antibody works very well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Shyambabu Chaurasiya

Verified customer

提交于 Jan 24 2018

Immunohistochemistry-Abreview for PFA fixed, paraffin embedded Goat anti-Rabbit HRP secondary antibody ab6721

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Very clear signal. The image is of a cross section of 3 metatarsal bones, primary antibody is Periostin (ab14041).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Haydee Gutierrez

Verified customer

提交于 Jan 09 2017

1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com