山羊抗兔IgG H&L (HRP) (ab205718)
Key features and details
- Goat Anti-Rabbit IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, WB, ELISA, IP
Related conjugates and formulations
概述
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产品名称
山羊抗兔IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
特异性
The antibody used for conjugation reacts with rabbit immunoglobulins of all classes. Cross-reactions as determined by ELISA for the unconjugated antibody (ab182016): Mouse IgG, rat IgG, and chicken IgY, less than 2%. Human IgG, less than 7%. -
经测试应用
适用于: IHC-P, WB, ELISA, IPmore details -
免疫原
This information is proprietary to Abcam and/or its suppliers.
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175471)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175773)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)
- Goat Anti-Rabbit IgG H&L (ab182016)
- Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab205718于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/2000 - 1/50000.
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WB | (6) |
1/2000 - 1/50000.
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ELISA | (1) |
1/5000 - 1/20000.
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IP |
Use at an assay dependent concentration.
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说明 |
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IHC-P
1/2000 - 1/50000. |
WB
1/2000 - 1/50000. |
ELISA
1/5000 - 1/20000. |
IP
Use at an assay dependent concentration. |
图片
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All lanes : Anti-beta Actin antibody (ab8227) at 1 µg/ml
Lane 1 : Liver (Human) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab205718) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab8227 overnight at 4°C. Antibody binding was detected using ab205718, and visualised using ECL development solution ab133406.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC image of histone H4 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab177840 at 1/1000 dilution. An HRP-conjugated secondary (Ab205718, 1/20000 dilution) was used to detect the primary for 1hr at room temperature. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : Anti-beta Actin antibody (ab8227) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : ab205718 (Left Image) at 1/20,000 and a competitor secondary (Right Image) at 1/50,000. Notice the increased background of the competitor product.
Performed under reducing conditions.
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab8227 overnight at 4°C. Antibody binding was detected using ab205718 (Left Image) and a competitor secondary (Right Image), and visualised using ECL development solution ab133406.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC image of beta tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab6046 at 1/100 dilution. An HRP-conjugated secondary (Ab205718, 1/20000 dilution) was used to detect the primary for 1hr at room temperature. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC image of Ki67 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab15580 at 1/1000 dilution. An HRP-conjugated secondary (Ab205718, 1/20000 dilution) was used to detect the primary for 1hr at room temperature. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.
For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.
This data was developed using the unconjugated antibody (ab182016).
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Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.
This data was developed using the unconjugated antibody (ab182016).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1675)
ab205718 被引用在 1675 文献中.
- Jiang R et al. METTL3 stabilizes HDAC5 mRNA in an m6A-dependent manner to facilitate malignant proliferation of osteosarcoma cells. Cell Death Discov 8:179 (2022). WB, IHC-P ; Human . PubMed: 35396379
- Hu Y et al. Curcumol enhances cisplatin sensitivity of gastric cancer: involvement of microRNA-7 and the nuclear factor-kappa B/snail family transcriptional repressor 1 axis. Bioengineered 13:11668-11683 (2022). WB ; Human . PubMed: 35510522
- Shen H et al. N6-methyladenosine (m6A) methyltransferase METTL3 regulates sepsis-induced myocardial injury through IGF2BP1/HDAC4 dependent manner. Cell Death Discov 8:322 (2022). WB ; Rat . PubMed: 35840562
- Zhang W et al. MicroRNA-744-5p is downregulated in colorectal cancer and targets SEPT2 to suppress the malignant phenotype. Mol Med Rep 23:N/A (2021). PubMed: 33200802
- Chen W et al. Downregulation of lncRNA ZFAS1 inhibits the hallmarks of thyroid carcinoma via the regulation of miR-302-3p on cyclin D1. Mol Med Rep 23:N/A (2021). PubMed: 33179076