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Immunology Immunoglobulins Heavy Chain IgG

山羊抗兔IgG H&L (HRP) (ab205718)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (1675)

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Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • ELISA - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • ELISA - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Key features and details

  • Goat Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, WB, ELISA, IP

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 6nm HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

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概述

  • 产品名称

    山羊抗兔IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 宿主

    Goat
  • 靶标种属

    Rabbit
  • 特异性

    The antibody used for conjugation reacts with rabbit immunoglobulins of all classes. Cross-reactions as determined by ELISA for the unconjugated antibody (ab182016): Mouse IgG, rat IgG, and chicken IgY, less than 2%. Human IgG, less than 7%.
  • 经测试应用

    适用于: IHC-P, WB, ELISA, IPmore details
  • 免疫原

    This information is proprietary to Abcam and/or its suppliers.

  • 偶联物

    HRP

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • 存储溶液

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Enzyme
    • HRP

相关产品

  • Alternative Versions

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175471)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175773)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)
    • Goat Anti-Rabbit IgG H&L (ab182016)
    • Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)
  • Related Products

    • Normal Goat Serum (ab7481)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab205718于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/2000 - 1/50000.
WB (6)
1/2000 - 1/50000.
ELISA (1)
1/5000 - 1/20000.
IP
Use at an assay dependent concentration.
说明
IHC-P
1/2000 - 1/50000.
WB
1/2000 - 1/50000.
ELISA
1/5000 - 1/20000.
IP
Use at an assay dependent concentration.

图片

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    All lanes : Anti-beta Actin antibody (ab8227) at 1 µg/ml

    Lane 1 : Liver (Human) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab205718) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 42 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab8227 overnight at 4°C. Antibody binding was detected using ab205718, and visualised using ECL development solution ab133406.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    IHC image of histone H4 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab177840 at 1/1000 dilution. An HRP-conjugated secondary (Ab205718, 1/20000 dilution) was used to detect the primary for 1hr at room temperature. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    All lanes : Anti-beta Actin antibody (ab8227) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : ab205718 (Left Image) at 1/20,000 and a competitor secondary (Right Image) at 1/50,000. Notice the increased background of the competitor product.

    Performed under reducing conditions.

    Observed band size: 42 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab8227 overnight at 4°C. Antibody binding was detected using ab205718 (Left Image) and a competitor secondary (Right Image), and visualised using ECL development solution ab133406.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    IHC image of beta tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab6046 at 1/100 dilution. An HRP-conjugated secondary (Ab205718, 1/20000 dilution) was used to detect the primary for 1hr at room temperature. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    IHC image of Ki67 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab15580 at 1/1000 dilution. An HRP-conjugated secondary (Ab205718, 1/20000 dilution) was used to detect the primary for 1hr at room temperature. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ELISA - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    ELISA - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.

    This data was developed using the unconjugated antibody (ab182016).

  • ELISA - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    ELISA - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

    This data was developed using the unconjugated antibody (ab182016).

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (1675)

发表研究结果有使用 ab205718?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab205718 被引用在 1675 文献中.

  • Jiang R  et al. METTL3 stabilizes HDAC5 mRNA in an m6A-dependent manner to facilitate malignant proliferation of osteosarcoma cells. Cell Death Discov 8:179 (2022). WB, IHC-P ; Human . PubMed: 35396379
  • Hu Y  et al. Curcumol enhances cisplatin sensitivity of gastric cancer: involvement of microRNA-7 and the nuclear factor-kappa B/snail family transcriptional repressor 1 axis. Bioengineered 13:11668-11683 (2022). WB ; Human . PubMed: 35510522
  • Shen H  et al. N6-methyladenosine (m6A) methyltransferase METTL3 regulates sepsis-induced myocardial injury through IGF2BP1/HDAC4 dependent manner. Cell Death Discov 8:322 (2022). WB ; Rat . PubMed: 35840562
  • Zhang W  et al. MicroRNA-744-5p is downregulated in colorectal cancer and targets SEPT2 to suppress the malignant phenotype. Mol Med Rep 23:N/A (2021). PubMed: 33200802
  • Chen W  et al. Downregulation of lncRNA ZFAS1 inhibits the hallmarks of thyroid carcinoma via the regulation of miR-302-3p on cyclin D1. Mol Med Rep 23:N/A (2021). PubMed: 33179076
View all Publications for this product

客户评价及客户问答

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1-8 of 8 Abreviews or Q&A

abreview for ab205718 - Goat Anti-Rabbit IgG H&L (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
We did the following experiments to test Goat Anti-Rabbit IgG H&L (HRP) as a secondary antibody.

Rat Tissue lysate - whole (cardiac muscle)
Loading amount: 20 µg
Specification: cardiac muscle
Blocking step: Milk as blocking agent for 1 hour (Concentration: 5 % · Temperature: 22°C)

Primary antibody: Anti-beta Actin antibody [SP124] (ab115777)
Dilution: 1/1000, Incubation time: 18 hours Temperature: 4°C.

Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Dilution: 1/5000, Incubation time: 1 hour Temperature: 22°C.
Detection method: ECL
Exposure: 2 minutes
Bands: Specific: (42 kDa)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 31 2022

Works well as a secondary Ab in ELISA

Excellent
Abreviews
Abreviews
abreview image
Application
ELISA
Recombinant protein was incubated with a rabbit-raised primary antibody to generate a standard curve in order to quantify unknown samples. Detection was possible even on low amounts of protein and consistent between experiments.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 16 2021

EXCELLENT SECONDARY ANTIBODY for WB

Excellent
Abreviews
Abreviews
Application
Western blot
EXCELLENT SECONDARY ANTIBODY for WB
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 10 2020

Anti-rabbit secondary successful IHC-P

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry paraffin embedded sections
The first image was incubated with ab40763 and ab205718 (at 1/200)
The second image is a negative control, with no primary antibody and ab205718 (at /200).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 08 2020

ab205718 tested by WB application

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Assay performed on whole cell lysate (mouse embryonic stem cells). Samples were mixed with 2X Laemli sample buffer and loaded on a 8% acrylamide gel together with Spectra™ Multicolor High Range Protein Ladder. Run performed in vertical tank at 100V for 1 hour followed by transfer in a wet system for 2 hours at 60V. The membrane was preblocked with 5% milk and then incubated with primary antibody (alpha-tubulin) overnight diluted in 5%milk. After washes the membrane was incubated with ab205718 1:2000 for 2 hours at RT. Bands were detected with ECL.
First lane:ladder, second lane:wild type mouse ES cells (D3), third lane: E-cadherin knock-out mouse embryonic cell line.
ab205718 worked for the chosen protein by WB. However, additional unspecific band detected.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Fabiana Luise

Verified customer

提交于 Jul 24 2019

Goat Anti-Rabbit IgG with AMPK alpha antibody (ab32047)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
- 20ug of bovine liver lysates were loaded on to 4-15% gradient gel.
- Blocking in 5% skim milk for an hour at room temperature.
- 1:2000 AMPK alpha antibody (ab32047) were incubated over night @ 4C
- 1:20000 secondary antibody (ab205718) used for an hour at room temperature.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Julie Kim

Verified customer

提交于 Apr 20 2017

NR1D1 Western blot

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
10ug whole cell lysate (human ES cells).
Blocking buffer - 5%BSA/1%OvAlb/PBS for 3 hours.
Primary antibody - NR1D1(ab174309) 1:1000, 15 hours at 4C, expected size 67kDa.
Secondary diluted in TBST (1:5000) for 3 hours at room temperature.
ECL prime: 1 second exposure.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 12 2016

Test ab205718 - Goat Anti-Rabbit IgG H&L (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Excellent specificity.
Excellent sensitivity.
WB conditions:
- Cell lisates: 20 μg protein
- non-reducing
- Primary antibody: Rabbit anti-LaminB1, ab133741. Abcam. Dilution 1:5000
- Secondary antibody: 1:6000
- Developer: ECL Prime, Amersham, GE Helathcare Life Sciences, Product code RPN2232
- Exposure time: 1 second, 2nd film out 4
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 01 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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