山羊抗兔IgG H&L (Cy5 ®)预吸附二抗(ab6564)
Key features and details
- Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Fr
Related conjugates and formulations
概述
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产品名称
山羊抗兔IgG H&L (Cy5 ®)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
经测试应用
适用于: Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Frmore details -
最小交叉反应
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
免疫原
Rabbit IgG, whole molecule
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偶联物
Cy5 ®. Ex: 650nm, Em: 667nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis. -
共轭说明
Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG -
克隆
多克隆 -
同种型
IgG -
常规说明
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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研究领域
应用
应用 | Ab评论 | 说明 |
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Flow Cyt | (1) |
Use at an assay dependent dilution.
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ICC/IF |
1/1000 - 1/5000.
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ELISA |
1/10000 - 1/50000.
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IHC-P |
Use at an assay dependent concentration.
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|
IHC-Fr |
Use at an assay dependent concentration.
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说明 |
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Flow Cyt
Use at an assay dependent dilution. |
ICC/IF
1/1000 - 1/5000. |
ELISA
1/10000 - 1/50000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
图片
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Immunofluorescence stainings of human prostate tissues
Sections were double labeled for different PAK isoforms, together with calponin (marker for smooth muscle cells), pan-cytokeratin (marker for epithelial cells of glands), or tyrosine hydroxylase (TH, marker for catecholaminergic nerves). Yellow color in merged pictures may indicate colocalization of targets. Shown are representative stainings from series with tissues from n = 5 patients for each combination.
ab6908 was used as secondary antibody to visualize various rabbit anti-PAK primary antibodies (red).
(From Figure 2 of Hennenberg et al)
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ab11175 staining Histone H2A.X in mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 4% BSA for 2 hours at 37°C. Antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS/4% BSA/0.05% Triton X-100) for 48 hours at 4°C. A Cy5®-conjugated goat anti-rabbit IgG H&L preadsorbed (ab6564) (1/300) was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (75)
ab6564 被引用在 75 文献中.
- Lun J et al. Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders. J Neuroinflammation 20:87 (2023). PubMed: 36997969
- Fassler M et al. TREM2 Agonism with a Monoclonal Antibody Attenuates Tau Pathology and Neurodegeneration. Cells 12:N/A (2023). PubMed: 37296669
- Xu HK et al. Region-specific sympatho-adrenergic regulation of specialized vasculature in bone homeostasis and regeneration. iScience 26:107455 (2023). PubMed: 37680481
- Ou S et al. Tibial cortex transverse transport potentiates diabetic wound healing via activation of SDF-1/CXCR4 signaling. PeerJ 11:e15894 (2023). PubMed: 37727693
- Huang J et al. A 12-gene panel in estimating hormone-treatment responses of castration-resistant prostate cancer patients generated using a combined analysis of bulk and single-cell sequencing data. Ann Med 55:2260387 (2023). PubMed: 37729607