山羊抗兔IgG H&L (Cy3.5 ®)预吸附二抗(ab6941)
Key features and details
- Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed
- Conjugation: Cy3.5 ®. Ex: 581nm, Em: 596nm
- Host species: Goat
- Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA
概述
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产品名称
山羊抗兔IgG H&L (Cy3.5 ®)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details -
最小交叉反应
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
免疫原
Full length native Rabbit IgG (purified).
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偶联物
Cy3.5 ®. Ex: 581nm, Em: 596nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above -
共轭说明
Cy3.5 (Cyanine 3.5-OSu) (Molecular Weight 1286 daltons) Absorption Wavelength: 581 nm Emission Wavelength: 596 nm Fluorochrome/Protein Ratio: 9.3 moles Cy3.5 per mole of Goat IgG -
克隆
多克隆 -
常规说明
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| ICC |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent dilution.
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| ELISA |
1/10000 - 1/50000.
|
| 说明 |
|---|
|
ICC/IF
1/1000 - 1/5000. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent dilution. |
|
ELISA
1/10000 - 1/50000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (5)
ab6941 被引用在 5 文献中.
- Jiang Z et al. Detection of CSTF2 by nano fluorescent probe and its correlation with malignant biological characteristics in liver cancer. Am J Cancer Res 13:5577-5589 (2023). PubMed: 38058835
- Mohapatra S et al. Power of an Organic Electron Acceptor in Modulation of Intracellular Mitochondrial Reactive Oxygen Species: Inducing JNK- and Caspase-Dependent Apoptosis of Cancer Cells. ACS Omega 6:7815-7828 (2021). PubMed: 33778293
- Weingarten G et al. Insulin receptor plays a central role in skin carcinogenesis by regulating cytoskeleton assembly. FASEB J 33:2241-2251 (2019). PubMed: 30332298
- Ramalho AF et al. Dietary fats promote functional and structural changes in the median eminence blood/spinal fluid interface-the protective role for BDNF. J Neuroinflammation 15:10 (2018). IHC-P ; Mouse . PubMed: 29316939
- Fioravante M et al. Inhibition of hypothalamic leukemia inhibitory factor exacerbates diet-induced obesity phenotype. J Neuroinflammation 14:178 (2017). PubMed: 28865476
