山羊抗兔IgG H&L (Cy3 ®)预吸附二抗(ab6939)
Key features and details
- Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISA
概述
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产品名称
山羊抗兔IgG H&L (Cy3 ®)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISAmore details -
最小交叉反应
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
免疫原
Full length native Rabbit IgG (purified).
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偶联物
Cy3 ®. Ex: 552nm, Em: 565nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. -
共轭说明
Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 9.9 moles Cy3 per mole of Goat IgG -
克隆
多克隆 -
同种型
IgG -
常规说明
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labelled without unwanted cross reaction.
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研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
1/1000 - 1/5000.
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| IHC-Fr | (1) |
Use at an assay dependent dilution.
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| IHC-P |
1/100. PubMed: 17045908
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| ICC |
Use at an assay dependent dilution.
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| Flow Cyt |
1/500 - 1/2500.
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| ELISA |
1/10000 - 1/50000.
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| 说明 |
|---|
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ICC/IF
1/1000 - 1/5000. |
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IHC-Fr
Use at an assay dependent dilution. |
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IHC-P
1/100. PubMed: 17045908 |
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ICC
Use at an assay dependent dilution. |
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Flow Cyt
1/500 - 1/2500. |
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ELISA
1/10000 - 1/50000. |
图片
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)Bockor et al PLoS One. 2014 Aug 5;9(8):e103954. doi: 10.1371/journal.pone.0103954. eCollection 2014. Fig 8. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Histone modifications associated with NOR-1 and NOR-2 loci in root tip cycling cells of Q.robur.
IFF detection of H3K9ac (A), H3K4me3 (B), H3K9me1 (C) and H3K27me2 (D). Green signals correspond to immunofluorescence of histone antibodies and red signals represent 18S rDNA FISH signals. Letter “n” marks the nucleoli. Scale bar is 5 µm.
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)Islam et al PLoS One. 2015 Jun 8;10(6):e0128306. doi: 10.1371/journal.pone.0128306. eCollection 2015. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Photomicrographs showing immunostaining of (Panel A) ABCG2 (red), (Panel B) Bmi-1 (green), (Panel C) C/EBPδ (red), (Panel D) PCNA (red), (Panel E) CK18 (green), and (Panel F) cleaved caspase-3 (red) in HOK (human oral keratinocytes) cells cultured for five days without subsequent storage (control).
Photomicrographs are representative of four independent samples.
Cell nuclei were counterstained with DAPI (blue).
Original magnification: 200x.
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)Buchholz et al PLoS One. 2015 Apr 7;10(4):e0122946. doi: 10.1371/journal.pone.0122946. eCollection 2015. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Examples of the different antibody stainings for functional analyses.
Left Panel: Ki-67 staining (Cy-3, red), HEK-293 transfected with NKX2-5-YFP (green).
Right Panel: Cyclin B staining (Cy-3, red), PANC-1 transfected with PRKCZ-YFP (green).
Original magnifications are indicated in the images.
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939) -
Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)Lane M: 3 µL Molecular ladder.
Lane 1: Rabbit IgG whole molecule (p/n 011-0102).
Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105).
Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103).
Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107).
Lane 5: Normal Rabbit Serum (p/n B309).
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
Primary Antibody: ab6939 used at 1/1000 for 60 min at RT.
Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody used at 1/40000 in blocking buffer for 30 min at RT.
Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.
Rabbit F(c) migrates slightly higher.
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Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)Lane 1: Rabbit IgG.
Load: 50 ng per lane.
Secondary antibody: ab6939 at 1/1000 for 60 min at RT. Blocked for 30 min at RT.
Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Rabbit IgG.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (236)
ab6939 被引用在 236 文献中.
- Woike D et al. The Shank/ProSAP N-Terminal (SPN) Domain of Shank3 Regulates Targeting to Postsynaptic Sites and Postsynaptic Signaling. Mol Neurobiol 61:693-706 (2024). PubMed: 37656313
- Li XJ et al. Allicin ameliorates sepsis-induced acute kidney injury through Nrf2/HO-1 signaling pathway. J Nat Med 78:53-67 (2024). PubMed: 37668824
- Kotarska K et al. Long-run real-time PCR analysis of repetitive nuclear elements as a novel tool for DNA damage quantification in single cells: an approach validated on mouse oocytes and fibroblasts. J Appl Genet 65:181-190 (2024). PubMed: 38110826
- Ma H et al. Disparate macrophage responses are linked to infection outcome of Hantan virus in humans or rodents. Nat Commun 15:438 (2024). PubMed: 38200007
- You Y et al. Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy. Nat Biomed Eng N/A:N/A (2023). PubMed: 36635419