山羊抗兔IgG H&L (Biotin) (ab207995)
Key features and details
- Goat Anti-Rabbit IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, ICC/IF, IP, Flow Cyt, WB, IHC-Fr, IHC-P
Related conjugates and formulations
概述
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产品名称
山羊抗兔IgG H&L (Biotin)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
特异性
The antibody used for conjugation reacts with rabbit immunoglobulins of all classes. Cross-reactions as determined by ELISA for the unconjugated antibody (ab182016): Mouse IgG, rat IgG, and chicken IgY, less than 2%. Human IgG, less than 7%. -
经测试应用
适用于: ELISA, ICC/IF, IP, Flow Cyt, WB, IHC-Fr, IHC-Pmore details -
免疫原
The details of the immunogen for this antibody are not available.
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偶联物
Biotin
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175471)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175773)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)
- Goat Anti-Rabbit IgG H&L (ab182016)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab207995于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
1/20000 - 1/200000.
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
1/500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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ELISA
1/20000 - 1/200000. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
1/500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)
IHC image of Histone H4 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. Ab207995 Goat Anti-Rabbit IgG H & L (Biotin) was used as the secondary antibody.
Staining was performed on a Leica BondTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab177840, 1/100 dilution, for 15 mins at room temperature, followed by ab207995, 1/2000 dilution, for 15 mins at room temperature. Detection was via an HRP conjugated ABC system and DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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ab207995 was tested by direct ELISA, where wells were coated with serially diluted rabbit IgG (1000 – 16 ng/ml) for 2 hours, followed by a 2 hour blocking step (5% BSA). ab207995 (1:20,000 dilution; 2 hours) was added and detected by streptavidin-HRP (ab7403; 1:10,000 dilution; 1 hour). Signal was developed by TMB substrate. Data from duplicates; +/- SD.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)
IHC image of beta Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. Ab207995 Goat Anti-Rabbit IgG H & L (Biotin) was used as the secondary antibody.
Staining was performed on a Leica BondTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab6046, 1/100 dilution, for 15 mins at room temperature, followed by ab207995, 1/1000 dilution, for 15 mins at room temperature. Detection was via an HRP conjugated ABC system and DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.
For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.
This data was developed using the unconjugated antibody (ab182016).
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Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.
This data was developed using the unconjugated antibody (ab182016).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab207995 被引用在 16 文献中.
- Weckbach LT et al. Association of Complement and MAPK Activation With SARS-CoV-2-Associated Myocardial Inflammation. JAMA Cardiol 7:286-297 (2022). PubMed: 34910083
- Na JR et al. Citric Acid-Enriched Extract of Ripe Prunus mume (Siebold) Siebold & Zucc. Induces Laxative Effects by Regulating the Expression of Aquaporin 3 and Prostaglandin E2 in Rats with Loperamide-Induced Constipation. J Med Food 25:12-23 (2022). PubMed: 35029511
- Karki A et al. Plexin-B2 and Semaphorins Do Not Drive Rhabdomyosarcoma Proliferation or Migration. Sarcoma 2022:9646909 (2022). PubMed: 35570846
- Li X et al. IGFBP7 remodels the tumor microenvironment of esophageal squamous cell carcinoma by activating the TGFβ1/SMAD signaling pathway. Oncol Lett 24:251 (2022). PubMed: 35761941
- Diaz Diaz AC et al. Preclinical Evaluation of Fingolimod in Rodent Models of Stroke With Age or Atherosclerosis as Comorbidities. Front Pharmacol 13:920449 (2022). PubMed: 35910379