Goat Anti-Rabbit IgG H&L (HRP)

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-Histone H3 (phospho S10) antibody (<a href='/products/primary-antibodies/histone-h3-phospho-s10-antibody-ab5176'>ab5176</a>) at 1 µg/mL

Lanes 1, 3 and 5:

untreated histones at 0.5 µg

Lanes 2, 4 and 6:

colcemid treated histones at 0.5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 15-20 kDa

true

Exposure time: 10s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (<a href='/products/primary-antibodies/beta-actin-antibody-loading-control-ab8227'>ab8227</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells at 20 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) cells at 20 µg

Lane 3:

Fish Liver at 20 µg

Lane 4:

Rabbit Liver at 20 µg

Lane 5:

MDCK (Canine kidney cell line) cells at 20 µg

Lane 6:

EBTr (cow trachea) cells at 20 µg

Lane 7:

SL-29 (chicken day 11 embryo) cells at 20 µg

Lane 8:

CHO (Chinese hamster ovary cell line) cells at 20 µg

Lane 9:

Xenopus laevis embryo at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 41 kDa

Observed band size: 41.7 kDa

false

Exposure time: 30s

• WB

AbReview64376****

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Primary antibody incubated for 15 hours at 4°C in TBST.

3% BSA used as blocking agent for 1 hour at 23°C.

All lanes:

Western blot - Anti-TGF beta Receptor I antibody (<a href='/products/primary-antibodies/tgf-beta-receptor-i-antibody-ab31013'>ab31013</a>) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

A2780 (Human ovarian cancer cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/10000 dilution

Predicted band size: 55 kDa

true

Exposure time: 15s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Secondary antibody - goat anti-rabbit H&L (HRP) (ab6721)

All lanes:

Western blot - Anti-CD45 antibody (<a href='/products/primary-antibodies/cd45-antibody-ab10558'>ab10558</a>) at 1/500 dilution

Lane 1:

Jurkat Whole Cell Lysate at 20 µg

Lane 2:

Jurkat Whole Cell Lysate at 20 µg with Human CD45 peptide (<a href='/products/unavailable/human-cd45-peptide-ab17553'>ab17553</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 147 kDa

false

Exposure time: 3min

• WB

Project

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-VDAC1/Porin + VDAC2 + VDAC3 antibody – Mitochondrial Loading Control (<a href='/products/primary-antibodies/vdac1-porin-vdac2-vdac3-antibody-mitochondrial-loading-control-ab15895'>ab15895</a>) at 1 µg/mL

Lane 1:

Hela Nuclear lysate at 20 µg

Lane 2:

Hela cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Lane 5:

HEK293 cell lysate at 20 µg

Lane 6:

Hela Nuclear lysate at 20 µg with Human VDAC1/Porin peptide (<a href='/products/unavailable/human-vdac1porin-peptide-ab16131'>ab16131</a>)

Lane 7:

Hela cell lysate at 20 µg with Human VDAC1/Porin peptide (<a href='/products/unavailable/human-vdac1porin-peptide-ab16131'>ab16131</a>)

Lane 8:

A431 cell lysate at 20 µg with Human VDAC1/Porin peptide (<a href='/products/unavailable/human-vdac1porin-peptide-ab16131'>ab16131</a>)

Lane 9:

Jurkat cell lysate at 20 µg with Human VDAC1/Porin peptide (<a href='/products/unavailable/human-vdac1porin-peptide-ab16131'>ab16131</a>)

Lane 10:

HEK293 cell lysate at 20 µg with Human VDAC1/Porin peptide (<a href='/products/unavailable/human-vdac1porin-peptide-ab16131'>ab16131</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 31 kDa

Observed band size: 31 kDa

true

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-p21 antibody [EPR362] (<a href='/products/primary-antibodies/p21-antibody-epr362-ab109520'>ab109520</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HUVEC cell lysate at 10 µg

Lane 4:

LnCap cell lysate at 10 µg

Lane 5:

U87 MG cell lysate at 10 µg

Lane 6:

293T cell lsyate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

Predicted band size: 18 kDa

false

• WB

AbReview81245****

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Western blot analysis using ab62352 at 1 : 1000 on Human iPSC-cardiomyocytes. Blocking agent and dilution buffer was 5% Skim Milk in TBS-Tween.

A cellular fractionation in Human iPSC-cardiomyocyte cells was performed to separate the nucleus from the cytoplasm (Lane 2).

All lanes:

Western blot - Anti-Nrf2 antibody [EP1808Y] (<a href='/products/primary-antibodies/nrf2-antibody-ep1808y-ab62352'>ab62352</a>) at 1/1000 dilution

Lane 1:

Human iPSC-cardiomyocytes cytoplasmatic fraction, at 20 µg

Lane 2:

Human iPSC-cardiomyocytes nuclear fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Observed band size: 100 kDa

false

• WB

PubMed

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

https : //www.sciencedirect.com/journal/biochemical-and-biophysical-research-communications

All lanes:

Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (<a href='/products/primary-antibodies/dfna5-gsdme-antibody-epr19859-n-terminal-ab215191'>ab215191</a>) at 1/1000 dilution

Lane 1:

Wild Type SGC-7901 (human papillomavirus-related endocervical adenocarcinoma cell line) cell lysates

Lane 2:

DFNA5/GSDME Knockout SGC-7901 cell lysates

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Predicted band size: 54 kDa

false

Image from Wang Y et al., Biochem Biophys Res Commu. 2018;495(1):1418-1425. Fig 4(A).; 10.1016/j.bbrc.2017.11.156 with permission from Elsevier.

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Western blot using ab4171 against Hela Nuclear Extract.

The expected band size for Apc7 is 63 kd, which corresponds to the band seen in this western. The band is blocked by the immunising peptide.

Secondary ab : Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000)
Exposure time : 1min

Lane 1 and 2 : 20μg/lane HeLa Nuclear

Lane 1 : ab4171 (1/500)
Lane 2 : ab4171 (1/500) + 1.0μg ab4171 peptide

All lanes:

Western blot - Anti-Apc7 antibody (<a href='/products/primary-antibodies/apc7-antibody-ab4171'>ab4171</a>)

Lane 1:

HeLa Nuclear Extract at 20 µg

Lane 2:

HeLa Nuclear Extract at 20 µg with Apc7 peptide (<a href='/products/unavailable/apc7-peptide-ab13734'>ab13734</a>)

Secondary

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Lane 2:

Goat polyclonal to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution

Predicted band size: 66 kDa

Observed band size: 39 kDa,45 kDa,63 kDa

false

Exposure time: 1min

• WB

AbReview23240****

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-MCP1 antibody (<a href='/products/primary-antibodies/mcp1-antibody-ab9669'>ab9669</a>) at 1/4000 dilution

All lanes:

lysate prepared from human smooth muscle treated with 100µg/ml glycated LDL at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 14-Dec kDa

true

Exposure time: 1min

Image courtesy of Dr Laura Toma by Abreview.

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Secondary antibody - anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (<a href='/products/primary-antibodies/hsp60-antibody-mitochondrial-marker-ab46798'>ab46798</a>) at 1/50000 dilution

All lanes:

Western blot - T-47D whole cell lysate (<a href='/products/cell-lysates/t-47d-whole-cell-lysate-ab14899'>ab14899</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Predicted band size: 61 kDa

Observed band size: 60 kDa

false

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Rabbit polyclonal to hnRNPA1 (ab4791) at 1/500.

Lane 1 : HeLa Whole Cell Extract
Lane 2 : HeLa Nuclear Extract

Secondary ab : Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000)

All lanes:

Western blot - Anti-hnRNP A1 antibody (<a href='/products/primary-antibodies/hnrnp-a1-antibody-ab4791'>ab4791</a>) at 1/500 dilution

Lane 1:

HeLa Whole Cell Extract

Lane 2:

HeLa Nuclear Extract

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Predicted band size: 38 kDa

Observed band size: 39 kDa

false

• WB

AbReview17936****

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Blocking Step : 5% Milk for 1 hour at 25°C.

All lanes:

Western blot - Anti-P70 S6 Kinase (phospho T389) antibody (<a href='/products/primary-antibodies/p70-s6-kinase-phospho-t389-antibody-ab2571'>ab2571</a>) at 1/250 dilution

Lane 1:

Normal Rat liver whole tissue lysate. at 20 µg

Lane 2:

Rat liver whole tissue lysate 24 hours after 90% partial hepatectomy treated with AEE788 (50 mg/kg). at 20 µg

Lane 3:

Rat liver whole tissue lysate 1 week after 90% partial hepatectomy treated with AEE788 (50 mg/kg). at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 59 kDa

Observed band size: 70 kDa

true

This image is courtesy of an anonymous Abreview

• WB

AbReview62868****

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (<a href='/products/primary-antibodies/padi4-pad4-antibody-epr20706-ab214810'>ab214810</a>) at 1/1000 dilution

Lane 1:

Wild-type mouse embryonic stem cells (E14) at 40 µg with BSA

Lanes 2 - 3:

mouse embryonic stem cells (E14) PADI4 Knock out at 40 µg with BSA

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 74 kDa

true

Exposure time: 18hr

This image is courtesy of an abreview by Maria Christophorou

• WB

AbReview50301****

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-RUNX2 antibody (<a href='/products/primary-antibodies/runx2-antibody-ab23981'>ab23981</a>) at 1/1000 dilution

Lane 1:

Bone tissue (tibia) from 17 weeks old Wistar male rats treated with doxorubicin hydrochloride injections for 7 weeks

Lane 2:

Bone tissue (tibia) from 20 weeks old Wistar female rats

Lane 3:

Bone tissue (tibia) from 56 weeks old ooforectomized Wistar female rats

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Exposure time: 40s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

ab19903 detects a band of the expected size (32kDa) in both mouse and rat heart lysate. This band is quenched completely by the addition of the immunizing peptide in the mouse lysate and is partially quenched by the addition of ab20431 in the rat heart lysate.

All lanes:

Western blot - Anti-Syntenin antibody (<a href='/products/primary-antibodies/syntenin-antibody-ab19903'>ab19903</a>) at 1 µg/mL

Lane 1:

Mouse heart lysate at 20 µg

Lane 2:

Rat heart lysate at 20 µg

Lane 3:

Mouse heart lysate at 20 µg with Mouse Syntenin peptide (<a href='/products/unavailable/mouse-syntenin-peptide-ab20431'>ab20431</a>)

Lane 4:

Rat heart lysate at 20 µg with Mouse Syntenin peptide (<a href='/products/unavailable/mouse-syntenin-peptide-ab20431'>ab20431</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/50000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa,50 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

ab6721 was used at dilution 1/100 with the primary antibody ab11370 in IHC-P. See the review on ab11370.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Anti-phospho-tau immunostaining in Dryocopus lineatus.

Tau-positivity in the midbrain (Panel A (shown) and B) and the corpus callosum (C) of the Dryocopus lineatus brain. The axonal tract staining demonstrates a thread-like pattern, similar to that seen with Gallyas sliver staining. Occasional intracellular tau-accumulations were identified within neurons (D).

For full method, please see paper.

Farah et al PLoS One. 2018 Feb 2;13(2):e0191526. doi: 10.1371/journal.pone.0191526. eCollection 2018. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• IHC-P

AbReview50581****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Immunohistochemical analysis of PFA-fixed paraffin-embedded rat cardiac tissue sections, labeling Conexin 43 with ab117843 at a dilution of 1/500 incubated for 12 hours at 4°C in 1% BSA in TBS. Antigen retrival was via Tris-EDTA pH 9.0 (heat mediated). Blocking was 3% BSA incubated for 1 hour at 37°C. The secondary was ab6721 at 1/500.

Image is courtesy of an Abreview submitted by Dr Helder Fonseca.

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Rabbit polyclonal to RAD54 (ab10705) at 1/500.

Lane 1 Marker
Lane 2 HeLa nuclear extract
Lane 3 HeLa cell lysate
Lane 4 A432 cell lysate
Lane 5 MCF7 cell lysate
Lane 6 HEK 293 cell lysate

Secondary antibody - Goat polyclonal to rabbit IgG (HRP) - ab6721.

Lane 1:

Western blot - Anti-RAD54 antibody (<a href='/products/primary-antibodies/rad54-antibody-ab10705'>ab10705</a>)

Lanes 2 - 6:

Western blot - Anti-RAD54 antibody (<a href='/products/primary-antibodies/rad54-antibody-ab10705'>ab10705</a>) at 1/500 dilution

Lane 1:

Marker

Lane 2:

HeLa nuclear cell lysate

Lane 3:

HeLa cell lysate

Lane 4:

A432 cell lysate

Lane 5:

MCF7 cell lysate

Lane 6:

Hek 293 cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

Predicted band size: 84 kDa

Observed band size: 70 kDa

false

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

ab5819 is specific for Histone H3 phosphorylated at residue S10 and tri methylated at residue K9. The activity of the antibody is blocked by the addition of the immunizing peptide, ab15644 (lanes 3 and 4).

All lanes:

Western blot - Anti-Histone H3 (tri methyl K9, phospho S10) antibody (<a href='/products/primary-antibodies/histone-h3-tri-methyl-k9-phospho-s10-antibody-ab5819'>ab5819</a>) at 1/500 dilution

Lanes 1, 5, 7 and 9:

HeLa histones at 0.5 µg

Lanes 2, 6, 8 and 10:

Colcemid-treated HeLa histones at 0.5 µg

Lane 3:

HeLa histones at 0.5 µg with Human Histone H3 (tri methyl K9, phospho S10) peptide (<a href='/products/unavailable/human-histone-h3-tri-methyl-k9-phospho-s10-peptide-ab15644'>ab15644</a>)

Lane 4:

Colcemid-treated HeLa histones at 0.5 µg with Human Histone H3 (tri methyl K9, phospho S10) peptide (<a href='/products/unavailable/human-histone-h3-tri-methyl-k9-phospho-s10-peptide-ab15644'>ab15644</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 22 kDa,60 kDa

false

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-CBX1 / HP1 beta antibody (<a href='/products/primary-antibodies/cbx1-hp1-beta-antibody-ab10478'>ab10478</a>) at 1 µg/mL

Lanes 1 and 6:

HeLa whole cell lysate at 20 µg

Lanes 2 and 7:

HeLa nuclear lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Lanes 4 and 9:

MCF7 cell lysate at 20 µg

Lanes 5 and 10:

HEK293 cell lysate at 20 µg

Lane 8:

A431 lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 21 kDa

Observed band size: 25 kDa,37 kDa,47 kDa

false

• WB

Project900****

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-acetyl-k18-antibody-chip-grade-ab1191'>ab1191</a>) at 1 µg/mL

Lane 1:

Histone prep at 0.5 µg/mL

Lane 2:

Histone prep at 0.5 µg/mL with Human Histone H3 (acetyl K18) peptide (<a href='/products/unavailable/human-histone-h3-acetyl-k18-peptide-ab24003'>ab24003</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Lane 1 - 2 : EEA1 antibody - Early Endosome Marker (ab2900) at 1/500 dilution, HEK293 Whole Cell lysate at 20 ug Lane 1 : as above Lane 2 : EEA1 peptide (ab14946) at 1 ug Secondary Goat polyclonal to Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution Performed under reducing conditions. Predicted band size : 160kD Observed band size : 180kD (why is the actual band size different from the predicted?)

All lanes:

Western blot - Anti-EEA1 antibody - Early Endosome Marker (<a href='/products/primary-antibodies/eea1-antibody-early-endosome-marker-ab2900'>ab2900</a>) at 1/500 dilution

Lane 1:

HEK293 Whole Cell lysate at 20 µg

Lane 2:

HEK293 Whole Cell lysate at 20 µg with Human EEA1 peptide (<a href='/products/unavailable/human-eea1-peptide-ab14946'>ab14946</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 162 kDa

Observed band size: 180 kDa

false

• WB

AbReview10746****

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-LC3B antibody - Autophagosome Marker (<a href='/products/primary-antibodies/lc3b-antibody-autophagosome-marker-ab48394'>ab48394</a>) at 1/2000 dilution

Lane 1:

Rat whole tissue lysate - Normal liver at 30 µg

Lane 2:

Rat whole tissue lysate - liver treated with AEE788 at 50 mg/kg 3 times a week for 1 week at 30 µg

Lane 3:

Rat whole tissue lysate - liver treated with RAD at 2.5 mg/kg daily for 1 week at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Lanes 1, 3 and 4:

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (<a href='/products/primary-antibodies/rna-polymerase-ii-ctd-repeat-ysptsps-phospho-s5-antibody-ab5131'>ab5131</a>) at 1/500 dilution

Lane 2:

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (<a href='/products/primary-antibodies/rna-polymerase-ii-ctd-repeat-ysptsps-phospho-s5-antibody-ab5131'>ab5131</a>) at 1/2000 dilution

Lanes 1 - 3:

HeLa nuclear extract at 20 µg

Lane 4:

HeLa nuclear extract at 20 µg with <em>S. cerevisiae</em> RNA polymerase II CTD repeat YSPTSPS peptide (<a href='/products/unavailable/s-cerevisiae-rna-polymerase-ii-ctd-repeat-ysptsps-peptide-ab12795'>ab12795</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 217 kDa

Observed band size: 250 kDa

false

Exposure time: 30s

• WB

Project335****

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (<a href='/products/primary-antibodies/beta-actin-antibody-loading-control-ab8227'>ab8227</a>) at 1/1000 dilution

Lanes 1 and 6:

HeLa nuclear lysate at 20 µg

Lanes 2 and 7:

HeLa whole cell lysate at 20 µg

Lanes 3 and 8:

A431 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Lanes 5 and 10:

HEK 293 cell lysate at 20 µg

Lane 9:

Jurkate cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 41 kDa

Observed band size: 41.7 kDa

false

Exposure time: 5s

• WB

Project

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

ab9053 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 5 where the activity of ab9053 is specifically blocked by the addition of the immunizing peptide (ab17567).

All lanes:

Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h4-tri-methyl-k20-antibody-chip-grade-ab9053'>ab9053</a>) at 1 µg/mL

Lanes 1, 5, 6 and 7:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Human Histone H4 peptide (<a href='/products/unavailable/human-histone-h4-peptide-ab2622'>ab2622</a>)

Lane 3:

Calf thymus histone lysate with Human Histone H4 (mono methyl K20) peptide (<a href='/products/unavailable/human-histone-h4-mono-methyl-k20-peptide-ab17043'>ab17043</a>)

Lane 4:

Calf thymus histone lysate with Human Histone H4 (di methyl K20) peptide (<a href='/products/unavailable/human-histone-h4-di-methyl-k20-peptide-ab14964'>ab14964</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

false

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-Cyclophilin B antibody - Loading Control (<a href='/products/primary-antibodies/cyclophilin-b-antibody-loading-control-ab16045'>ab16045</a>) at 1 µg/mL

Lane 1:

HeLa nuclear lysate at 20 µg

Lane 2:

HeLa whole cell lysate at 20 µg

Lane 3:

A431 whole cell lysate at 20 µg

Lane 4:

Jurkat whole cell lysate at 20 µg

Lane 5:

HEK293 whole cell lysate at 20 µg

Lane 6:

HeLa nuclear lysate at 20 µg with Human Cyclophilin B peptide (<a href='/products/unavailable/human-cyclophilin-b-peptide-ab16277'>ab16277</a>)

Lane 7:

HeLa whole cell lysate at 20 µg with Human Cyclophilin B peptide (<a href='/products/unavailable/human-cyclophilin-b-peptide-ab16277'>ab16277</a>)

Lane 8:

A431 whole cell lysate at 20 µg with Human Cyclophilin B peptide (<a href='/products/unavailable/human-cyclophilin-b-peptide-ab16277'>ab16277</a>)

Lane 9:

Jurkat whole cell lysate at 20 µg with Human Cyclophilin B peptide (<a href='/products/unavailable/human-cyclophilin-b-peptide-ab16277'>ab16277</a>)

Lane 10:

HEK293 whole cell lysate at 20 µg with Human Cyclophilin B peptide (<a href='/products/unavailable/human-cyclophilin-b-peptide-ab16277'>ab16277</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 24 kDa

Observed band size: 21 kDa

false

Exposure time: 30s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Lanes 1 & 3 : Sumo 2+3 antibody (ab3742) at 1/500 dilution
Lanes 2 & 4 : Sumo 2+3 antibody (ab3742) at 1/1000 dilution
Lanes 1-4 : HeLa nuclear extract at 20 ug

Lane 1 : as above
Lane 2 : as above
Lane 3 : Sumo 2+3 peptide (ab13760) at 1 ug/ml
Lane 4 : Sumo 2+3 peptide (ab13760) at 1 ug/ml

SecondaryGoat polyclonal to Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution developed using the ECL technique

Performed under reducing conditions.

Exposure time : 1 minute

Predicted band sizes : 11.6 & 10.

Lanes 1 and 3:

Western blot - Anti-Sumo 2 + Sumo 3 antibody (<a href='/products/primary-antibodies/sumo-2-sumo-3-antibody-ab3742'>ab3742</a>) at 1/500 dilution

Lanes 2 and 4:

Western blot - Anti-Sumo 2 + Sumo 3 antibody (<a href='/products/primary-antibodies/sumo-2-sumo-3-antibody-ab3742'>ab3742</a>) at 1/1000 dilution

Lanes 1 - 2:

HeLa nuclear extract at 20 µg

Lanes 3 - 4:

HeLa nuclear extract at 20 µg with Human Sumo 2 + Sumo 3 peptide (<a href='/products/unavailable/human-sumo-2-sumo-3-peptide-ab13760'>ab13760</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Predicted band size: 12 kDa

Observed band size: 15 kDa,18 kDa

true

Exposure time: 1min

• WB

Project729****

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-Cyclophilin B antibody - Loading Control (<a href='/products/primary-antibodies/cyclophilin-b-antibody-loading-control-ab16045'>ab16045</a>) at 1 µg/mL

Lane 1:

Rat Liver at 20 µg

Lane 2:

Mouse 3T3 at 20 µg

Lane 3:

Dog at 20 µg

Lane 4:

Chicken at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 24 kDa

Observed band size: 25 kDa

false

Exposure time: 30s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h2az-antibody-chip-grade-ab4174'>ab4174</a>) at 1/500 dilution

All lanes:

Calf thymus histone lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 14 kDa

Observed band size: 15 kDa

false

• WB

Project

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

ab4074 cross-reacts strongly with Mouse 3T3 lysate and shows weak cross-reactivity with Rat liver, Cow, Chicken and CHO lysates.

All lanes:

Western blot - Anti-alpha Tubulin antibody - Loading Control (<a href='/products/primary-antibodies/alpha-tubulin-antibody-loading-control-ab4074'>ab4074</a>) at 1 µg/mL

Lane 1:

Mouse 3T3 lysate at 20 µg

Lane 2:

Rat liver lysate at 20 µg

Lane 3:

Cow cell lysate at 20 µg

Lane 4:

Chicken cell lysate at 20 µg

Lane 5:

CHO lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

true

Exposure time: 30s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-CD81 antibody [EPR4244] (<a href='/products/primary-antibodies/cd81-antibody-epr4244-ab109201'>ab109201</a>) at 1/1000 dilution

Lane 1:

Ramos cell lysate at 10 µg

Lane 2:

JAR cell lysate at 10 µg

Lane 3:

U87-MG cell lysate at 10 µg

Lane 4:

IM-9 cell lysate at 10 µg

Lane 5:

Peripheral blood mononuclear cell (PBMC) cell lysate at 10 µg

Lane 6:

C6 cell lysate at 10 µg

Lane 7:

PC-12 cell lysate at 10 µg

Lane 8:

RAW264.7 cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

Predicted band size: 25 kDa

false

• WB

Project

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (<a href='/products/primary-antibodies/beta-actin-antibody-loading-control-ab8227'>ab8227</a>) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate at 20 µg

Lanes 2 and 7:

HeLa whole cell lysate at 20 µg

Lane 3:

A431 (human epidermoid carcinoma cell line) cell lysate at 20 µg

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 20 µg

Lane 5:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 20 µg

Lane 6:

HeLa nuclear lysate at 20 µg

Lane 8:

A431 cell lysate at 20 µg

Lane 9:

Jurkat cell lysate at 20 µg

Lane 10:

HEK-293 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 41 kDa

Observed band size: 41.7 kDa

false

Exposure time: 5s

• WB

PubMed

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215191).

https : //www.sciencedirect.com/journal/biochemical-and-biophysical-research-communications

All lanes:

Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (<a href='/products/primary-antibodies/dfna5-gsdme-antibody-epr19859-bsa-and-azide-free-ab223877'>ab223877</a>) at 1/1000 dilution

Lane 1:

Wild Type SGC-7901 cell lysates

Lane 2:

DFNA5/GSDME Knockout SGC-7901 cell lysates

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Predicted band size: 54 kDa

false

Image from Wang Y et al., Biochem Biophys Res Commu. 2018;495(1):1418-1425. Fig 4(A).; 10.1016/j.bbrc.2017.11.156 with permission from Elsevier.

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

ab8895 is specific for mono-methylated Lysine 4 of histone H3 and does not recognize di- or tri-methyl Lysine 4 nor methylation at Lysine 9. This is shown in lane 2 where the activity of the antibody is specifically blocked by the addition of the immunizing peptide (ab1340).

All lanes:

Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (<a href='/products/primary-antibodies/histone-h3-mono-methyl-k4-antibody-chip-grade-ab8895'>ab8895</a>) at 1/500 dilution

All lanes:

Calf thymus histone lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 18 kDa

false

Exposure time: 2min

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-beta Tubulin antibody - Loading Control (<a href='/products/primary-antibodies/beta-tubulin-antibody-loading-control-ab6046'>ab6046</a>) at 1/500 dilution

Lanes 1 and 5:

HeLa Cell lysate at 20 µg

Lanes 2 and 6:

A431 Cell lysate at 20 µg

Lanes 3 and 7:

MCF7 Cell lysate at 20 µg

Lanes 4 and 8:

293 Cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 49 kDa

Observed band size: 55 kDa

true

Exposure time: 10s

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

All lanes:

Western blot - Anti-VCAM1 antibody [EPR5047] (<a href='/products/primary-antibodies/vcam1-antibody-epr5047-ab134047'>ab134047</a>) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

HuT 78 cell lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Lane 4:

Mouse brain tissue lysate at 10 µg

Lane 5:

Mouse kidney tissue lysate at 10 µg

Lane 6:

Mouse spleen tissue lysate at 10 µg

Lane 7:

Rat brain tissue lysate at 10 µg

Lane 8:

Rat kidney tissue lysate at 10 µg

Lane 9:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Predicted band size: 81 kDa

false

• WB

Lab

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab1791 overnight at 4°C.

Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody was used for detection.

Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (<a href='/products/primary-antibodies/histone-h3-antibody-nuclear-marker-and-chip-grade-ab1791'>ab1791</a>) at 1/1000 dilution

Lanes 1 and 4:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lanes 2 and 5:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg

Lanes 3 and 6:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 10s

• WB

AbReview

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721)

Blocked with 5% non-fat milk for 1 hour at 18°C

All lanes:

Anti-NEK7 antibody (<a href='/products/unavailable/nek7-antibody-ab80948'>ab80948</a>) at 1/2000 dilution

All lanes:

Mouse brain tissue cytoplasmic lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

true

Exposure time: 10s

This image is courtesy of an Abreview submitted by Maryna Polyakova.