AB175695

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed

Name: 山羊抗兔IgG H&L (Alexa Fluor® 568)预吸附二抗 (ab175695)| Abcam中文官网
Brand: Abcam Limited
SKU: AB175695
Price: 2231 CNY
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(21 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 21 publications.

查看别名

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

3 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (AB175695)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (AB175695)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab175695 Alexa Fluor® 568 Goat anti-Rabbit IgG (H&L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® 568 is pseudocolored in yellow.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (AB175695)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175695 Alexa Fluor® 568 Goat anti-Rabbit IgG (H&L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® 568 is pseudocolored in yellow.

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (AB175695)

Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (AB175695)

关键信息

宿主种属

Goat

靶标种属

Rabbit

靶标亚型

IgG

靶标特异性

Heavy & Light chains

最低交叉反应

Mouse, Rat, Human

预吸附

Yes

偶联物

Alexa Fluor® 568

激发波长/发射波长

Ex: 578nm, Em: 603nm

应用

ELISA, Flow Cyt, IHC-Fr, ICC/IF, IHC-P

applications

克隆

Polyclonal

亚型

IgG

特异性

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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产品详情

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Antiserum was cross adsorbed using a human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

存储溶液

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information Rabbit IgG

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文献 (21)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 224: PubMed40880129

2025

ATG conjugation-dependent/independent mechanisms underlie lysosomal stress-induced TFEB regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Shiori Akayama,Takayuki Shima,Tatsuya Kaminishi,Mengying Cui,Jlenia Monfregola,Kohei Nishino,Andrea Ballabio,Hidetaka Kosako,Tamotsu Yoshimori,Shuhei Nakamura

Science advances 11:eadu8634 PubMed40668908

2025

A forward-engineered, muscle-driven soft robotic swimmer.

Applications

Unspecified application

Species

Unspecified reactive species

William Cartwright Drennan,Onur Aydin,Bashar Emon,Zhengwei Li,Md Saddam Hossain Joy,Alexandra Barishman,Yelim Kim,Margaret Wei,Danette Denham,Annika Carrillo,M Taher A Saif

Advanced healthcare materials 14:e2402757 PubMed39491520

2024

Intervening to Preserve Function in Ischemic Cardiomyopathy with a Porous Hydrogel and Extracellular Matrix Composite in a Rat Myocardial Infarction Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yasunari Hayashi,Taro Fujii,Seungil Kim,Takahiro Ozeki,Stephen F Badylak,Antonio D'Amore,Masato Mutsuga,William R Wagner

Journal of bacteriology 206:e0036724 PubMed39347571

2024

Development of inducible promoter and CRISPRi plasmids functional in .

Applications

Unspecified application

Species

Unspecified reactive species

Adam M Nock,Tina R Clark,Ted Hackstadt

The EMBO journal 43:2954-2978 PubMed38822137

2024

YIPF3 and YIPF4 regulate autophagic turnover of the Golgi apparatus.

Applications

Unspecified application

Species

Unspecified reactive species

Shinri Kitta,Tatsuya Kaminishi,Momoko Higashi,Takayuki Shima,Kohei Nishino,Nobuhiro Nakamura,Hidetaka Kosako,Tamotsu Yoshimori,Akiko Kuma

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305217 PubMed38029345

2023

Ferritin-Based Nanocomposite Hydrogel Promotes Tumor Penetration and Enhances Cancer Chemoimmunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Liu,Qian Liang,Jia-Qi Luo,Yu-Xuan Li,Xin Zhang,Kelong Fan,Jin-Zhi Du

Acta biomaterialia 173:93-108 PubMed37977292

2023

Nuclear deformation regulates YAP dynamics in cancer associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Bashar Emon,M Saddam H Joy,Luke Lalonde,Anan Ghrayeb,Umnia Doha,Lauren Ladehoff,Reed Brockstein,Chaimongkol Saengow,Randy H Ewoldt,M Taher A Saif

Advanced biology 8:e2300127 PubMed37786311

2023

Human Kidney Proximal Tubule-Microvascular Model Facilitates High-Throughput Analyses of Structural and Functional Effects of Ischemia-Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Erin M Shaughnessey,Samuel H Kann,Joseph L Charest,Else M Vedula

Frontiers in neurology 14:1243301 PubMed37830095

2023

knock-out rats exhibit abundant spike-wave discharges in EEG, exacerbated with valproate treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyu Liu,Kazuyuki Fujihara,Yuchio Yanagawa,Hajime Mushiake,Tomokazu Ohshiro

Fluids and barriers of the CNS 20:66 PubMed37705104

2023

Proteomic alterations in the brain and blood-brain barrier during brain Aβ accumulation in an APP knock-in mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Shingo Ito,Ryotaro Yagi,Seiryo Ogata,Takeshi Masuda,Takashi Saito,Takaomi Saido,Sumio Ohtsuki

View all publications

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed