山羊抗兔IgG H&L (Alexa Fluor® 405)预吸附二抗
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed
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(9 Publications)
Suitable for ELISA, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.
查看别名
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175654)
ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175654 Alexa Fluor® 405 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L, pre-adsorbed, used at 1μg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
- Alexa Fluor®
Unknown
Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175654)
反应性数据
产品详情
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能和储存信息
形式
纯化工艺
纯化说明
存储溶液
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
文献 (9)
Recent publications for all applications. Explore the full list and refine your search
Nature neuroscience 28:2201-2216 PubMed41044342
2025
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Science advances 9:eadd6480 PubMed37595032
2023
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Molecular neurobiology 60:1766-1781 PubMed36571720
2022
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Glia 70:106-122 PubMed34498776
2021
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Molecular cell 79:934-949.e14 PubMed32822587
2020
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Reproductive biology and endocrinology : RB&E 17:41 PubMed31060561
2019
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Translational psychiatry 7:e1192 PubMed28786974
2017
Applications
IHC-Fr
Species
Human
Stem cells and development 26:964-972 PubMed28376684
2017
Applications
Flow Cyt
Species
Horse
Stem cell research & therapy 8:80 PubMed28420427
2017
Applications
Unspecified application
Species
Unspecified reactive species
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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