山羊抗小鼠IgG+IgM H&L (HRP)预吸附二抗(ab47827)
Key features and details
- Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ELISA, WB, IHC-P, IHC-Fr, Electron Microscopy
概述
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产品名称
山羊抗小鼠IgG+IgM H&L (HRP)预吸附二抗
参阅全部 IgG + IgM 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, Mouse IgG and Mouse IgM. -
经测试应用
适用于: ICC/IF, ELISA, WB, IHC-P, IHC-Fr, Electron Microscopymore details -
最小交叉反应
Cow, Horse, Human more details -
免疫原
Full length native proteins (purified) (Mouse)
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偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
IgG and protease free -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
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- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
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- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47827于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/2500.
|
|
| ELISA |
1/5000 - 1/40000.
|
|
| WB |
1/2000 - 1/10000.
|
|
| IHC-P |
1/500 - 1/2500.
|
|
| IHC-Fr |
1/500 - 1/2500.
|
|
| Electron Microscopy |
Use at an assay dependent dilution.
|
| 说明 |
|---|
|
ICC/IF
1/500 - 1/2500. |
|
ELISA
1/5000 - 1/40000. |
|
WB
1/2000 - 1/10000. |
|
IHC-P
1/500 - 1/2500. |
|
IHC-Fr
1/500 - 1/2500. |
|
Electron Microscopy
Use at an assay dependent dilution. |
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (22)
ab47827 被引用在 22 文献中.
- Zhu M et al. lncRNA LINC00284 promotes nucleus pulposus cell proliferation and ECM synthesis via regulation of the miR-205-3p/Wnt/ß-catenin axis. Mol Med Rep 25:N/A (2022). PubMed: 35322864
- Wu M et al. YTHDF2 interference suppresses the EMT of cervical cancer cells and enhances cisplatin chemosensitivity by regulating AXIN1. Drug Dev Res 83:1190-1200 (2022). PubMed: 35489084
- Hashimoto K et al. Clinicopathological assessment of cancer/testis antigens NY‑ESO‑1 and MAGE‑A4 in osteosarcoma. Eur J Histochem 66:N/A (2022). PubMed: 35736245
- Han XD et al. miRNA-124 regulates palmitic acid-induced epithelial-mesenchymal transition and cell migration in human retinal pigment epithelial cells by targeting LIN7C. Exp Ther Med 24:481 (2022). PubMed: 35761801
- Han H et al. miR-199b-3p contributes to acquired resistance to cetuximab in colorectal cancer by targeting CRIM1 via Wnt/ß-catenin signaling. Cancer Cell Int 22:42 (2022). PubMed: 35090460
