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山羊抗小鼠IgG3 heavy chain (HRP) (ab97260)

Submit a review Q&A (1)References (16)

Key features and details

  • Goat Anti-Mouse IgG3 heavy chain (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

概述

  • 产品名称

    山羊抗小鼠IgG3 heavy chain (HRP)
    参阅全部 IgG3 二抗

  • 宿主

    Goat

  • 靶标种属

    Mouse

  • 特异性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG3. Cross reactivity with other immunoglobulins is less than 2%.

  • 经测试应用

    适用于: ICC, IHC-P, ELISA, WBmore details

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

  • 共轭说明

    Molar enzyme/ antibody protein ratio is 4:1

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab97260于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (as Primary)
WB
1/2000 - 1/25000. Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000
说明
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (as Primary)
WB
1/2000 - 1/25000. Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (16)

发表研究结果有使用 ab97260？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab97260 被引用在 16 文献中.

  • Kovalenko AO  et al. Vaccine Candidate Against COVID-19 Based on Structurally Modified Plant Virus as an Adjuvant. Front Microbiol 13:845316 (2022). PubMed: 35295298
  • Kong XW  et al. Vesicular Stomatitis Virus-Based Epstein-Barr Virus Vaccines Elicit Strong Protective Immune Responses. J Virol 96:e0033622 (2022). PubMed: 35404082
  • Lv Y  et al. Identification of B-cell dominant epitopes in the recombinant protein P29 from Echinococcus granulosus. Immun Inflamm Dis 10:e611 (2022). PubMed: 35478448
  • Schmidt C  et al. A taRNA vaccine candidate induces a specific immune response that protects mice against Chikungunya virus infections. Mol Ther Nucleic Acids 28:743-754 (2022). PubMed: 35664702
  • Plum LA  et al. Antibody production in mice requires neither vitamin D, nor the vitamin D receptor. Front Immunol 13:960405 (2022). PubMed: 36341456
View all Publications for this product

客户评价及客户问答

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Question

I have some specifictechnical questions regarding your secondary antibodies:

1) What level of cross-reactivity is there between thefollowing four antibodies(ie how specific for their IgG subclass target):

mouse mAb anti human IgG1-HRP #ab99774
mouse mAb anti human IgG2-HRP #ab99784
mouse mAb anti human IgG3-HRP #ab99829
mouse mAb anti human IgG4-HRP #ab99817

2) Of the following pairs of goat anti mouse secondary antibodies, which would have the least level of cross-reactivity (ie most specific for its target):

non-pre-adsorbedvs preadsorbed
anti IgM ab97230vs ab98679
anti IgG1 ab98693vsab97240
anti IgG2a ab97245 vs ab98698
anti IgG2b ab97250 vs ab98703
anti IgG2c ab97255 vs ab98722
anti IgG3 ab97260vsab98708


3) What bulk discount can you offer if we wish to purchase antibodies from you with list price totalling over £1000 ?

Many thanks,

Tapan Bhattacharyya
London School of Hygiene & Tropical Medicine

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Answer

Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these to you when I receive them.

For the second question, each antibody of each pair is the same with regards to isotype specificity but differ with regards to species specificity. Please note that you have reversed the two anti-IgG1 antibodies in your list: ab98693 is pre-adsorbed for species reactivity, and ab97240 is not.

anti IgM ab97230 vs ab98679 - Same with respect to isotype specificity. (No reactivity with other isotypes was detected). Cross-adsorbed to remove reactivity with human and rat IgG. (Less than 1% reactivity).

anti IgG1 ab98693 vs ab97240 - ab98693 is pre-adsorbed for species reactivity, and ab97240 is not. As above, regarding isotype specificity and species reactivity.

anti IgG2a ab97245 vs ab98698 - As above, regarding isotype specificity and species reactivity.

anti IgG2b ab97250 vs ab98703 - As above, regarding isotype specificity and species reactivity.

anti IgG2c ab97255 vs ab98722 - As above, regarding isotype specificity and species reactivity.

anti IgG3 ab97260 vs ab98708 - As above, regarding isotype specificity and species reactivity.

Our bulk discount is based on number of units purchased, not price. The discount begins at 5% for 5-9 units, 10% for 10-19 units, and so on. A proforma invoice will have the exact amount of the discount, and we will be happy to provide a proforma before you place your order.

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