山羊抗小鼠IgG H&L (TRITC) (ab6786)
Key features and details
- Goat Anti-Mouse IgG H&L (TRITC)
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA
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概述
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产品名称
山羊抗小鼠IgG H&L (TRITC)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details -
免疫原
Mouse IgG whole molecule
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偶联物
TRITC. Ex: 547nm, Em: 572nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Related Products
应用
应用 | Ab评论 | 说明 |
---|---|---|
Immunomicroscopy |
Use at an assay dependent concentration.
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Flow Cyt |
1/2000 - 1/4000.
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|
IHC-P |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
ICC/IF |
1/1000 - 1/5000. (PubMed:15659422)
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ELISA |
1/10000 - 1/50000.
|
说明 |
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Immunomicroscopy
Use at an assay dependent concentration. |
Flow Cyt
1/2000 - 1/4000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
1/1000 - 1/5000. (PubMed:15659422) |
ELISA
1/10000 - 1/50000. |
图片
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Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (TRITC) (ab6786) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 561nm laser and 610/20 bandpass filter.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (50)
ab6786 被引用在 50 文献中.
- Qi X et al. Shp2 suppresses fat accumulation in white adipose tissue by activating Wnt/ß-catenin signaling following vertical sleeve gastrectomy in obese rats with type-2 diabetes. Exp Ther Med 23:302 (2022). PubMed: 35340882
- Xu L et al. Galectin-3 Enhances Osteogenic Differentiation of Precursor Cells From Patients With Diffuse Idiopathic Skeletal Hyperostosis via Wnt/β-Catenin Signaling. J Bone Miner Res 37:724-739 (2022). PubMed: 35064940
- Zhang C et al. Narrow H3K4me2 is required for chicken PGC formation. J Cell Physiol 236:1391-1400 (2021). PubMed: 32749682
- Lee G et al. Broad-Spectrum Antiviral Activity of 3D8, a Nucleic Acid-Hydrolyzing Single-Chain Variable Fragment (scFv), Targeting SARS-CoV-2 and Multiple Coronaviruses In Vitro. Viruses 13:N/A (2021). PubMed: 33918914
- Zhao R et al. Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells. Nat Commun 12:2989 (2021). PubMed: 34017000