山羊抗小鼠IgG H&L (HRP) (ab6789)
Key features and details
- Goat Anti-Mouse IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB
概述
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产品名称
山羊抗小鼠IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details -
免疫原
mouse IgG whole molecule
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 6.5
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
MOUSE IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Horseradish Peroxidase (HRP) -
克隆
多克隆 -
同种型
IgG -
常规说明
The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support. -
研究领域
相关产品
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Substrate reagent
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- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
1/500 - 1/2500.
|
|
IP |
Use at an assay dependent concentration. PubMed: 17164758
|
|
Dot blot |
Use at an assay dependent concentration.
|
|
ELISA |
1/100000.
|
|
IHC-P |
1/500 - 1/2500.
|
|
IHC-Fr |
1/500 - 1/2500.
|
|
Immunomicroscopy |
Use at an assay dependent concentration.
|
|
WB | (3) |
1/2000 - 1/10000.
|
说明 |
---|
ICC
1/500 - 1/2500. |
IP
Use at an assay dependent concentration. PubMed: 17164758 |
Dot blot
Use at an assay dependent concentration. |
ELISA
1/100000. |
IHC-P
1/500 - 1/2500. |
IHC-Fr
1/500 - 1/2500. |
Immunomicroscopy
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. |
图片
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IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8224, 3 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1/2000 dilution
All lanes : Cytosolic fraction prepared from murine brain tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 194 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute -
ab49969 Immunoprecipitating HA Tag in HEK-293T whole cell lysate. 25 µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1584)
ab6789 被引用在 1584 文献中.
- Fan M et al. IRE1α regulates the PTHrP-IHH feedback loop to orchestrate chondrocyte hypertrophy and cartilage mineralization. Genes Dis 11:464-478 (2024). PubMed: 37588212
- Bou T et al. Muscle fibre transition and transcriptional changes of horse skeletal muscles during traditional Mongolian endurance training. Equine Vet J 56:178-192 (2024). PubMed: 37345447
- Zhang XR et al. Effect of Silicone Patch Containing Metal-organic Framework on Hypertrophic Scar Suppression. In Vivo 38:235-245 (2024). PubMed: 38148076
- Chen J et al. MiRNA-26a-5p inhibits preterm labor initiation by targeting and regulating TRPC3 ion channel protein expression. Environ Toxicol 39:357-366 (2024). PubMed: 37755144
- Luo QQ et al. ZBTB20 mediates stress-induced visceral hypersensitivity via activating the NF-κB/transient receptor potential channel pathway. Neurogastroenterol Motil 36:e14718 (2024). PubMed: 38009899