山羊抗小鼠IgG H&L (HRP) (ab6789)
Key features and details
- Goat Anti-Mouse IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB
概述
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产品名称
山羊抗小鼠IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details -
免疫原
mouse IgG whole molecule
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Horseradish Peroxidase (HRP) -
克隆
多克隆 -
同种型
IgG -
常规说明
The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support. -
研究领域
相关产品
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应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
1/500 - 1/2500.
|
|
IP |
Use at an assay dependent concentration. PubMed: 17164758
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|
Dot blot |
Use at an assay dependent concentration.
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|
ELISA |
1/100000.
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|
IHC-P |
1/500 - 1/2500.
|
|
IHC-Fr |
1/500 - 1/2500.
|
|
Immunomicroscopy |
Use at an assay dependent concentration.
|
|
WB | (3) |
1/2000 - 1/10000. PubMed: 17413034
|
说明 |
---|
ICC
1/500 - 1/2500. |
IP
Use at an assay dependent concentration. PubMed: 17164758 |
Dot blot
Use at an assay dependent concentration. |
ELISA
1/100000. |
IHC-P
1/500 - 1/2500. |
IHC-Fr
1/500 - 1/2500. |
Immunomicroscopy
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. PubMed: 17413034 |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8224, 3 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1/2000 dilution
All lanes : Cytosolic fraction prepared from murine brain tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 194 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute -
Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.
ab49969 Immunoprecipitating HA Tag in HEK-293T whole cell lysate. 25 µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1046)
ab6789 被引用在 1046 文献中.
- Yang L et al. Nsun4 and Mettl3 mediated translational reprogramming of Sox9 promotes BMSC chondrogenic differentiation. Commun Biol 5:495 (2022). WB ; Rat . PubMed: 35614315
- Yin GN et al. Pericyte-derived extracellular vesicles-mimetic nanovesicles improves peripheral nerve regeneration in mouse models of sciatic nerve transection. Int J Mol Med 49:N/A (2022). PubMed: 34935051
- Li J & Gu H Paeonol suppresses lipid formation and promotes lipid degradation in adipocytes. Exp Ther Med 23:78 (2022). PubMed: 34938364
- Peng Y et al. Role and mechanism of the Dectin-1-mediated Syk/NF-?B signaling pathway in Talaromyces marneffei infection. Exp Ther Med 23:84 (2022). PubMed: 34938366
- Wang J et al. Knockdown of ETV4 promotes autophagy-dependent apoptosis in GBM cells by reducing the transcriptional activation of EMP1. Oncol Lett 23:41 (2022). PubMed: 34976153