山羊抗小鼠IgG H&L (FITC)预吸附二抗(ab7064)
Key features and details
- Goat Anti-Mouse IgG H&L (FITC) preadsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA
概述
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产品名称
山羊抗小鼠IgG H&L (FITC)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details -
最小交叉反应
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
免疫原
Full length native Mouse IgG (purified).
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偶联物
FITC. Ex: 493nm, Em: 528nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Related Products
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 15767251
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Immunomicroscopy |
Use at an assay dependent concentration.
|
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Flow Cyt | (1) |
1/1000 - 1/2000.
|
IHC-P |
Use at an assay dependent concentration.
|
|
IHC-Fr | (1) |
Use at an assay dependent concentration.
|
ELISA |
1/10000 - 1/50000.
|
说明 |
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ICC/IF
Use at an assay dependent concentration. PubMed: 15767251 |
Immunomicroscopy
Use at an assay dependent concentration. |
Flow Cyt
1/1000 - 1/2000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ELISA
1/10000 - 1/50000. |
图片
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)Humphries, W.H. 4th et al PLoS One. 2011;6(10):e26626. doi: 10.1371/journal.pone.0026626. Epub 2011 Oct 24 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Rab7 and LAMP1 are highly colocalized in BS-C-1 cells and in HeLa cells
LAMP1 was detected with a mouse antiLAMP1 antibody in ICC/IF analysis of BS-C-1 cells.
Anti-LAMP1 was visualized using ab7064 at 1/500 diulution.
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Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (FITC, pre-adsorbed) (ab7064) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (33)
ab7064 被引用在 33 文献中.
- Coppola L et al. KCTD15 Is Overexpressed in her2+ Positive Breast Cancer Patients and Its Silencing Attenuates Proliferation in SKBR3 CELL LINE. Diagnostics (Basel) 12:N/A (2022). PubMed: 35328144
- Ieni A et al. Immunoexpression of p62/SQSTM1/Sequestosome-1 in human primary and recurrent IDH1/2 wild-type glioblastoma: A pilot study. Oncol Lett 24:336 (2022). PubMed: 36039055
- Smaldone G et al. KCTD15 deregulation is associated with alterations of the NF-?B signaling in both pathological and physiological model systems. Sci Rep 11:18237 (2021). PubMed: 34521919
- Li C et al. LncRNA PVT1 Knockdown Ameliorates Myocardial Ischemia Reperfusion Damage via Suppressing Gasdermin D-Mediated Pyroptosis in Cardiomyocytes. Front Cardiovasc Med 8:747802 (2021). PubMed: 34595225
- Xu Z et al. Effects of shRNA-mediated silencing of PDE5A3 on intracellular cGMP and free Ca2+ levels and human prostate smooth muscle cell proliferation from benign prostatic hyperplasia. Exp Ther Med 21:322 (2021). PubMed: 33732295