山羊抗小鼠IgG H&L (FITC) (ab6785)
Key features and details
- Goat Anti-Mouse IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Dot blot, IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WB
Related conjugates and formulations
概述
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产品名称
山羊抗小鼠IgG H&L (FITC)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: Dot blot, IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WBmore details -
免疫原
mouse whole molecule
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偶联物
FITC. Ex: 493nm, Em: 528nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.01% BSA, 0.424% Potassium phosphate solution, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Goat Anti-Mouse IgG [H&L] FITC Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
应用 | Ab评论 | 说明 |
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Dot blot |
Use at an assay dependent concentration.
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IHC (PFA fixed) |
1/1000.
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Immunomicroscopy |
Use at an assay dependent concentration.
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|
Flow Cyt |
1/500 - 1/2500. PubMed: 17989178
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|
IHC-P |
Use at an assay dependent concentration.
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|
IHC-Fr |
Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365.
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ICC/IF | (1) |
1/1000 - 1/5000. (PMID16006199)
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ELISA |
1/10000 - 1/50000.
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WB |
1/10000. PubMed: 17217624
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说明 |
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Dot blot
Use at an assay dependent concentration. |
IHC (PFA fixed)
1/1000. |
Immunomicroscopy
Use at an assay dependent concentration. |
Flow Cyt
1/500 - 1/2500. PubMed: 17989178 |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365. |
ICC/IF
1/1000 - 1/5000. (PMID16006199) |
ELISA
1/10000 - 1/50000. |
WB
1/10000. PubMed: 17217624 |
图片
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785)This image is courtesy of an Abreview submitted by J Chai.
ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (536)
ab6785 被引用在 536 文献中.
- Fan W & Zhou J Icariside II protects dopaminergic neurons from 1‑methyl‑4‑phenylpyridinium‑induced neurotoxicity by downregulating HDAC2 to restore mitochondrial function. Exp Ther Med 27:40 (2024). PubMed: 38125349
- Zhang MW et al. Curcumin relieves oxaliplatin-induced neuropathic pain via reducing inflammation and activating antioxidant response. Cell Biol Int 48:872-882 (2024). PubMed: 38480956
- Srivastav S et al. Motor neuron activity enhances the proteomic stress caused by autophagy defects in the target muscle. PLoS One 19:e0291477 (2024). PubMed: 38166124
- Huang JM et al. CX3CL1/CX3CR1 Signaling Mediated Neuroglia Activation Is Implicated in the Retinal Degeneration: A Potential Therapeutic Target to Prevent Photoreceptor Death. Invest Ophthalmol Vis Sci 65:29 (2024). PubMed: 38231527
- Le Gall-Lanotto C et al. Road-traffic-related air pollution contributes to skin barrier alteration and growth defect of sensory neurons. Exp Dermatol 33:e15009 (2024). PubMed: 38284185