山羊抗小鼠IgG H&L (DyLight® 594)预吸附二抗(ab96881)
Key features and details
- Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed
- Conjugation: DyLight® 594. Ex: 593nm, Em: 618nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Flow Cyt
Related conjugates and formulations
概述
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产品名称
山羊抗小鼠IgG H&L (DyLight® 594)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
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经测试应用
适用于: ICC/IF, IHC-P, Flow Cytmore details -
最小交叉反应
Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat more details -
免疫原
Other Immunogen Type corresponding to Mouse IgG.
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偶联物
DyLight® 594. Ex: 593nm, Em: 618nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96881于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/50 - 1/500.
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IHC-P |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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说明 |
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ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
图片
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)
ab96881 was used at dilution 1/100 for IHC analysis of FFPE human kidney. Primary Antibody: mouse anti-p53 (clone DO-1) used at a dilution of 1/100. Secondary Antibody: Red-fluorescent goat anti-mouse IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated ab96881 (2 different lots ) used at a dilution of 1/100 (5µg/mL). Counterstain: DAPI (blue)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (22)
ab96881 被引用在 22 文献中.
- Wu N et al. Essential role of MALAT1 in reducing traumatic brain injury. Neural Regen Res 17:1776-1784 (2022). PubMed: 35017438
- Shi Y et al. ROS Promote Hypoxia-Induced Keratinocyte Epithelial-Mesenchymal Transition by Inducing SOX2 Expression and Subsequent Activation of Wnt/ß-Catenin. Oxid Med Cell Longev 2022:1084006 (2022). PubMed: 35035654
- Selinger M et al. Integrative RNA profiling of TBEV-infected neurons and astrocytes reveals potential pathogenic effectors. Comput Struct Biotechnol J 20:2759-2777 (2022). PubMed: 35685361
- Selinger M et al. Tick-borne encephalitis virus capsid protein induces translational shutoff as revealed by its structural-biological analysis. J Biol Chem 298:102585 (2022). PubMed: 36223838
- Wang W et al. Electrofusion Stimulation Is an Independent Factor of Chromosome Abnormality in Mice Oocytes Reconstructed via Spindle Transfer. Front Endocrinol (Lausanne) 12:705837 (2021). PubMed: 34413830