山羊抗小鼠IgG H&L (Cy2 ®)预吸附二抗(ab6944)
Key features and details
- Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed
- Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
- Host species: Goat
- Suitable for: ICC/IF, IHC-P, ELISA, WB, Flow Cyt, IHC-Fr
Related conjugates and formulations
Alexa Fluor® 405
Alexa Fluor® 488
Alexa Fluor® 555
Alexa Fluor® 568
Alexa Fluor® 594
Alexa Fluor® 647
Alexa Fluor® 680
Alexa Fluor® 750
Alexa Fluor® 790
Alkaline Phosphatase
APC
beta-galactosidase
Biotin
Cy3 ®
Cy5 ®
Cy5.5 ®
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 650
FITC
Glucose Oxidase
Gold 10nm
Gold 6nm
HRP
HRP polymer
PE
Texas Red ®
TRITC
Unconjugated
Unconjugated
概述
-
产品名称
山羊抗小鼠IgG H&L (Cy2 ®)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: ICC/IF, IHC-P, ELISA, WB, Flow Cyt, IHC-Frmore details -
最小交叉反应
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
免疫原
Full length native protein (purified) corresponding to IgG.
-
偶联物
Cy2 ®. Ex: 489nm, Em: 505nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. -
共轭说明
Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 5.1 moles Cy2 per mole of Goat IgG -
克隆
多克隆 -
常规说明
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
-
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ELISA |
1/10000 - 1/50000.
|
|
| WB |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
ELISA
1/10000 - 1/50000. |
|
WB
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
图片
-
Western blot - Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944)Secondary
Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944) at 1/1000 dilution (60 minutes at Room temperature)
Observed band size: 25, 55 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (11)
ab6944 被引用在 11 文献中.
- Saga Y et al. The cytosolic lncRNA dutA affects STATa signaling and developmental commitment in Dictyostelium. Genes Cells 28:111-128 (2023). PubMed: 36504347
- Umebayashi M et al. A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins. J Cell Biol 222:N/A (2023). PubMed: 36571579
- Beznoussenko GV et al. The Diffusion Model of Intra-Golgi Transport Has Limited Power. Int J Mol Sci 24:N/A (2023). PubMed: 36674888
- Collins C et al. Tubulin acetylation promotes penetrative capacity of cells undergoing radial intercalation. Cell Rep 36:109556 (2021). PubMed: 34407402
- Piao CS et al. Depression following traumatic brain injury in mice is associated with down-regulation of hippocampal astrocyte glutamate transporters by thrombin. J Cereb Blood Flow Metab 39:58-73 (2019). PubMed: 29135354