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山羊抗小鼠IgG H&L (Biotin)预吸附二抗(ab7067)

Submit a review Q&A (2)References (7)

Key features and details

  • Goat Anti-Mouse IgG H&L (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, WB, ELISA, Dot blot

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 10nm Gold 6nm HRP HRP polymer PE Texas Red ® TRITC Unconjugated Unconjugated

概述

  • 产品名称

    山羊抗小鼠IgG H&L (Biotin)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Mouse

  • 特异性

    No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

  • 经测试应用

    适用于: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, WB, ELISA, Dot blotmore details

  • 最小交叉反应


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native mouse IgG (purified).

  • 偶联物

    Biotin

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    Mouse Secondary Antibody conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7067于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/2000 - 1/10000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ELISA
1/100000 - 1/800000.
Dot blot
Use at an assay dependent concentration.
说明
ICC/IF
1/2000 - 1/10000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ELISA
1/100000 - 1/800000.
Dot blot
Use at an assay dependent concentration.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (7)

发表研究结果有使用 ab7067？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7067 被引用在 7 文献中.

  • Cui X  et al. Neuroprotective Effect of Optogenetics Varies With Distance From Channelrhodopsin-2 Expression in an Amyloid-ß-Injected Mouse Model of Alzheimer's Disease. Front Neurosci 14:583628 (2020). PubMed: 33162881
  • Liu L  et al. CP-31398 attenuates endometrial cancer cell invasion, metastasis and resistance to apoptosis by downregulating MDM2 expression. Int J Oncol 54:942-954 (2019). PubMed: 30628640
  • Wang KW  et al. Optogenetics-induced activation of glutamate receptors improves memory function in mice with Alzheimer's disease. Neural Regen Res 14:2147-2155 (2019). PubMed: 31397354
  • Lei X  et al. Diazoxide inhibits of ER stress-mediated apoptosis during oxygen-glucose deprivation in vitro and cerebral ischemia-reperfusion in vivo. Mol Med Rep 17:8039-8046 (2018). PubMed: 29693708
  • Lin L  et al. Cocaine- and amphetamine-regulated transcript (CART) is associated with dopamine and is protective against ischemic stroke. Mol Med Rep 18:3298-3304 (2018). PubMed: 30066844
  • Simon-O'Brien E  et al. Etifoxine improves sensorimotor deficits and reduces glial activation, neuronal degeneration, and neuroinflammation in a rat model of traumatic brain injury. J Neuroinflammation 13:203 (2016). PubMed: 27565146
  • Laurentino SS  et al. Regucalcin is broadly expressed in male reproductive tissues and is a new androgen-target gene in mammalian testis. Reproduction 142:447-56 (2011). PubMed: 21680783

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Question

What dilution do you suggest for ab7067 in IHC-P?

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Answer

Thank you for your recent telephone enquiry.

I am sorry it has taken a little time to confirm this information with the originator for you.

They suggest to use this antibody between 1:100 - 1:1000 in IHC-P. However, pleasethis is a guideline only and may require some optimization by the end user.

I hope this will be helpful to you, and that your preliminary experiment has gone well. If you have any further questions, please do not hesitate to contact us.

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Question

I am interested in obtaining some of your Biotinylated Goat anti Mouse IgG to use in a multiple step FACS staining for a dim surface antigen. I notice that you do not suggest the use of this Ab for FACS, is this because it does not work or because you have no data there?

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Answer

This antibody has never been tested specifically for FACS, therefore we can't guarantee results. However, since this antibody is a secondary, it will probably work well.

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