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山羊抗小鼠IgG H&L (Biotin) (ab97021)

Submit a review Q&A (2)References (14)

Key features and details

  • Goat Anti-Mouse IgG H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, ELISA, WB

概述

  • 产品名称

    山羊抗小鼠IgG H&L (Biotin)
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Mouse

  • 特异性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

  • 经测试应用

    适用于: IHC-P, ICC/IF, ELISA, WBmore details

  • 偶联物

    Biotin

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

  • 共轭说明

    Biotin/ antibody protein ratio is 4:1

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab97021于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/500 - 1/5000.
ICC/IF
1/200 - 1/1000.
ELISA
1/20000 - 1/400000.
WB
1/20000 - 1/400000. Predicted molecular weight: 36 kDa.
说明
IHC-P
1/500 - 1/5000.
ICC/IF
1/200 - 1/1000.
ELISA
1/20000 - 1/400000.
WB
1/20000 - 1/400000. Predicted molecular weight: 36 kDa.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (14)

发表研究结果有使用 ab97021？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab97021 被引用在 14 文献中.

  • Dicks AR  et al. Chondrogenic Differentiation of Human-Induced Pluripotent Stem Cells. Methods Mol Biol 2598:87-114 (2023). PubMed: 36355287
  • Pena MJ  et al. Unveiling the Metabolic Effects of Glycomacropeptide. Int J Mol Sci 22:N/A (2021). PubMed: 34575895
  • Liang Y  et al. Re-Differentiation of Human Meniscus Fibrochondrocytes Differs in Three-Dimensional Cell Aggregates and Decellularized Human Meniscus Matrix Scaffolds. Ann Biomed Eng 48:968-979 (2020). PubMed: 31147805
  • Huynh NPT  et al. High-depth transcriptomic profiling reveals the temporal gene signature of human mesenchymal stem cells during chondrogenesis. FASEB J 33:358-372 (2019). PubMed: 29985644
  • Adkar SS  et al. Step-Wise Chondrogenesis of Human Induced Pluripotent Stem Cells and Purification Via a Reporter Allele Generated by CRISPR-Cas9 Genome Editing. Stem Cells 37:65-76 (2019). PubMed: 30378731
View all Publications for this product

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Dear Technical Team, Hope this e-mail finds you well! Kindly arrange to send us the certificate of compliance (CoC) for the below Abcam products as desired by one of our customers : Cat No. Description ab97021 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) ab97049 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) ab97023 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) ab60314 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) ab97080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed ab65623 ECL Western Blotting Substrate Kit - 50 Tests ab52476 Rabbit monoclonal [EP1829Y] to CaMKII ab11800 Goat polyclonal to DCXR ab7260 Rabbit polyclonal to GFAP - Astrocyte Marker ab104224 Mouse monoclonal [1B7] to FOX3 ab76260 Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9) ab64230 Mounting Medium ab82477 Rabbit polyclonal to Adenosine A1 Receptor ab3462 Rabbit polyclonal to alpha 1 Adrenergic Receptor

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Answer

I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.

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Question

1) wie rein ist ab64255 (BSA?)? Ich moechte den AK radiomarkieren, biotin auch wichtig. 2) Konzentration/Volumen? "125ml at mg/ml" macht keinen Sinn.

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Answer

Vielen Dank für Ihren Anruf. ab64255 wurde über Affinitätschromatographie aus dem Antiserum aufgereinigt; der exakte Reinheitsgrade wurde leider nicht bestimmt. Der Puffer, in dem der Antikörper vorliegt, enthält BSA. Die Konzentration beträgt 7 µg/ml, und weil es sich um ein ready-to-use-Produkt handelt, bekommen Sie tatsächlich 125 ml ! Für Ihre Versuchsvorhaben könnte sich dieser Antikörper daher vielleicht nicht unbedingt eignen - weil sich 125 ml doch eher schlecht verarbeiten lassen. Gerne möchte ich Ihnen zwei Alternativen nennen, die in weniger Volumen erhältlich sind (ab97021 und ab97033). Beide Antikörper sind biotinyliert, ab97033 ist zusätzlich noch prae-absorbiert, d.h. er reagiert nicht mit den auf dem Datenblatt angegebenen Spezies (wie Huhn, Rind, Ziege etc): Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-Biotin-ab97021.html). Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-Biotin-preadsorbed-ab97033.html). Ich hoffe, diese Informationen helfen Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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