山羊抗小鼠IgG H&L (Biotin) (ab6788)

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

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Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

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There are a few differences between the two pairs of antibodies you have identified. A few of these include the purification used (affitiny vs IgG fraction) as well as the ratio of conjugation. The ab6788 and ab6720 is estimated to have a conjugation ratio of 10-20 per IgG molecule whilst ab97049 and ab97021 have a ratio of 1:4.

I am not familiar with the amplification technique you intend to use and cannot therefore anticipate how these differences may alter the results.

If you could share a more detailed description of the procedure I may be able to advise in more detail.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

Thank you for contacting Abcam.

I feel that both products are suitable for IHC-P. Both have many specific references and in-depth scientific support data right on the datasheet. They differ mainly in the species in which they were raised and in the tags used for detection. Ab6728 is an HRP conjugate whereas Ab6788 is Biotin labeled. I believe that you should choose the secondary which has an associated detection system that you are most familiar with in case you should run into any staining issues.

I hope this information is helpful. Please let me know if you have any questions.

Thank you very much again for your reply. I appreciate the time you have spent in the laboratory and understand your concerns. As it appears to me your biotin-labeled secondary and the appropriate detection system are indeed working and it is regrettable the results have not been successful with the Progerin antibody yet. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial of ab66587. I apologise for the inconvenience and am pleased to offer you a free of charge replacement (with either a new vial or any other primary antibody of your choice) or credit note in compensation. Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you very much for confirming these details. Before proceeding with this case, I would appreciate if you could confirm which detection system was used with the biotin-labeled secondary antibody ab6788: Have you used a HRP-conjugated anti-biotin antibody or HRP-conjugated streptavidin, and is this working for other primary antibodies in your lab? I look forward to your reply.

Thank you for your enquiry. Unfortunately, the molecular weight of this product is not specifically measured, but the molecular weight of non reduced IgG is approximately 150-160 kDa. The molecular weight of biotin is ~250 Daltons. Yes, there are 10-20 molecules of Biotin conjugated to the IgG. The ratio of 10-20 is based on the combination ratio and typical results from our standard conjugation protocol. Function and purity of intact conjugate is confirmed by Immuno-electrophoresis (with anti biotin) and ELISA against mouse IgG using a HRP conjugated streptavidin as a secondary reagent. I hope　this information is helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

Thank you for getting back to me. Phosphate buffered saline can be used as an antibody diluent as an alternative to TBS for western blot analysis. However, with regards storage I would recommend that the antibodies are alituotted and frozen at -20oC upon arrival without prior dilution. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.