山羊抗小鼠IgG H&L (Biotin) (ab6788)
Key features and details
- Goat Anti-Mouse IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ICC, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB
概述
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产品名称
山羊抗小鼠IgG H&L (Biotin)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: ICC/IF, ICC, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details -
免疫原
mouse IgG whole molecule
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偶联物
Biotin
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
克隆
多克隆 -
同种型
IgG -
常规说明
The conjugate is expected to have between 5 and 10 moles of biotin per mole of IgG.
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研究领域
应用
应用 | Ab评论 | 说明 |
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ICC/IF |
1/1000 - 1/5000.
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ICC |
Use at an assay dependent dilution. PubMed: 17980964
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Dot blot |
Use at an assay dependent concentration.
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|
ELISA |
1/350000.
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|
IHC-P |
Use at an assay dependent concentration.
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|
IHC-Fr |
Use at an assay dependent concentration.
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|
Immunomicroscopy |
Use at an assay dependent concentration.
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|
WB |
1/2000 - 1/20000.
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说明 |
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ICC/IF
1/1000 - 1/5000. |
ICC
Use at an assay dependent dilution. PubMed: 17980964 |
Dot blot
Use at an assay dependent concentration. |
ELISA
1/350000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Immunomicroscopy
Use at an assay dependent concentration. |
WB
1/2000 - 1/20000. |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (Biotin) (ab6788)
IHC image of Histone H1 staining in a section of formalin-fixed paraffin-embedded human normal colon*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was then incubated with ab11080, 1/1,000 dilution, for 15 mins at room temperature. A goat anti-mouse biotinylated secondary antibody (ab6788, 1/1000 dilution), was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10,000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10 min at room temperature. The section was then counterstained with hematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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ELISA - Goat Anti-Mouse IgG H&L (Biotin) (ab6788)
ELISA results of purified ab6788 tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated and TMB substrate.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (74)
ab6788 被引用在 74 文献中.
- Chen S et al. Immunomagnetic microscopy of tumor tissues using quantum sensors in diamond. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35082154
- Yamamoto K et al. Protocol for generating a mouse model of gastric MALT lymphoma and the identification of MALT lymphoma cell populations by immunostaining. STAR Protoc 3:101155 (2022). PubMed: 35146453
- Yao H et al. Fraxetin exerts anticancer effect in glioma by suppressing MiR-21-3p. Drug Dev Res 83:501-511 (2022). PubMed: 34523750
- Karki A et al. Plexin-B2 and Semaphorins Do Not Drive Rhabdomyosarcoma Proliferation or Migration. Sarcoma 2022:9646909 (2022). PubMed: 35570846
- Zhu M et al. DNA-PAINT Imaging Accelerated by Machine Learning. Front Chem 10:864701 (2022). PubMed: 35620648