山羊抗小鼠IgG H&L (Alkaline Phosphatase) (ab6790)
Key features and details
- Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, IHC-Fr
概述
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产品名称
山羊抗小鼠IgG H&L (Alkaline Phosphatase)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: ICC/IF, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, IHC-Frmore details -
免疫原
mouse IgG whole molecule
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偶联物
Alkaline Phosphatase
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Anti-Mouse Conjugated Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/200 - 1/1000.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| WB |
1/1000 - 1/4000.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ELISA |
1/15000.
|
|
| IHC-P |
1/200 - 1/1000.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
1/200 - 1/1000. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/4000. |
|
Dot blot
Use at an assay dependent concentration. |
|
ELISA
1/15000. |
|
IHC-P
1/200 - 1/1000. |
|
IHC-Fr
Use at an assay dependent concentration. |
图片
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Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6790)Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6790) at 1/1000 dilution + Mouse IgG
Observed band size: 28,55 kDa why is the actual band size different from the predicted?Incubated with ab6790 for 60 min at room temperature.
Blocked for 30 min at room temperature.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab6790 被引用在 8 文献中.
- Palomino J et al. Embryo Buoyancy and Cell Death Gene Expression During Embryogenesis of Yellow-Tail Kingfish Seriola lalandi. Front Cell Dev Biol 9:630947 (2021). PubMed: 33816479
- Trainor BM et al. Distinct requirements for the COMPASS core subunits Set1, Swd1, and Swd3 during meiosis in the budding yeast Saccharomyces cerevisiae. G3 (Bethesda) 11:N/A (2021). PubMed: 34849786
- Khalid H et al. Discovery of novel Hepatitis C virus inhibitor targeting multiple allosteric sites of NS5B polymerase. Infect Genet Evol 84:104371 (2020). PubMed: 32485331
- Popova S et al. Predominant synthesis of giant myofibrillar proteins in striated muscles of the long-tailed ground squirrel Urocitellus undulatus during interbout arousal. Sci Rep 10:15185 (2020). PubMed: 32938992
- Zhao X et al. MicroRNA-939-5p directly targets IGF-1R to inhibit the aggressive phenotypes of osteosarcoma through deactivating the PI3K/Akt pathway. Int J Mol Med 44:1833-1843 (2019). PubMed: 31545400
- Li X et al. Effects of in Utero Exposure to Dicyclohexyl Phthalate on Rat Fetal Leydig Cells. Int J Environ Res Public Health 13:N/A (2016). IHC . PubMed: 26907321
- Anttila K et al. Variation in temperature tolerance among families of Atlantic salmon (Salmo salar) is associated with hypoxia tolerance, ventricle size and myoglobin level. J Exp Biol 216:1183-90 (2013). PubMed: 23487268
- Huang HY et al. The embryonic expression patterns and the knockdown phenotypes of zebrafish ADP-ribosylation factor-like 6 interacting protein gene. Dev Dyn 238:232-40 (2009). PubMed: 19097054