山羊抗小鼠IgG H&L (ab6708)
Key features and details
- Goat Anti-Mouse IgG H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WB
Related conjugates and formulations
Agarose
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Alkaline Phosphatase
APC
beta-galactosidase
Biotin
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DyLight® 488
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FITC
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Gold 10nm
Gold 6nm
HRP
HRP polymer
IRDye® 680RD
PE
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TRITC
Unconjugated
概述
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产品名称
山羊抗小鼠IgG H&L
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WBmore details -
免疫原
Mouse IgG, whole molecule
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
1/150000.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| Immunodiffusion |
Use at an assay dependent concentration.
|
|
| Conjugation |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| WB |
1/3000 - 1/15000.
|
| 说明 |
|---|
|
ELISA
1/150000. |
|
IP
Use at an assay dependent concentration. |
|
Immunodiffusion
Use at an assay dependent concentration. |
|
Conjugation
Use at an assay dependent concentration. |
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
WB
1/3000 - 1/15000. |
图片
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Western blot - Goat Anti-Mouse IgG H&L (ab6708)Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution + Mouse IgG at 0.1 µg
Secondary
Secondary antibody: DyLight™ 800 goat secondary antibody at 1/20000 dilution
Observed band size: 28,55 kDa why is the actual band size different from the predicted?ab6708 incubation overnight at 4°C.
Both heavy (55kDa) and light (28kDa) chains are detected.
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Western blot - Goat Anti-Mouse IgG H&L (ab6708)All lanes : Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution
Lane 1 : Mouse IgG (whole molecule)
Lane 2 : Mouse IgG Fc fragment
Lane 3 : Mouse IgG Fab fragment
Lane 4 : Mouse IgM kappa
Lane 5 : Mouse serum
Lysates/proteins at 0.05 µg per lane.
Secondary
All lanes : HRP Donkey Anti-Goat IgG at 1/40000 dilutionab6708 incubated for 1 hour at room temperature.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (49)
ab6708 被引用在 49 文献中.
- Kim SH et al. GlycoGrip: Cell Surface-Inspired Universal Sensor for Betacoronaviruses. ACS Cent Sci 8:22-42 (2022). PubMed: 35106370
- Dong Z et al. Increased expression of SRPK1 (serine/arginine-rich protein-specific kinase 1) is associated with progression and unfavorable prognosis in cervical squamous cell carcinoma. Bioengineered 13:6100-6112 (2022). PubMed: 35192432
- Maywald ML et al. Rap1 Activity Is Essential for Focal Adhesion and Slit Diaphragm Integrity. Front Cell Dev Biol 10:790365 (2022). PubMed: 35372328
- Huang Y et al. Parkinson's Disease Derived Exosomes Aggravate Neuropathology in SNCA*A53T Mice. Ann Neurol 92:230-245 (2022). PubMed: 35596947
- Schürmann H et al. Analysis of monocyte cell tractions in 2.5D reveals mesoscale mechanics of podosomes during substrate-indenting cell protrusion. J Cell Sci 135:N/A (2022). PubMed: 35621127