山羊抗小鼠IgG H&L (ab6708)
Key features and details
- Goat Anti-Mouse IgG H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WB
Related conjugates and formulations
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Alkaline Phosphatase
APC
beta-galactosidase
Biotin
Cy2 ®
Cy3 ®
Cy5 ®
Cy5.5 ®
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 650
FITC
Glucose Oxidase
Gold 10nm
Gold 6nm
HRP
HRP polymer
PE
Texas Red ®
TRITC
Unconjugated
概述
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产品名称
山羊抗小鼠IgG H&L
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WBmore details -
免疫原
Mouse IgG, whole molecule
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Secondary Antibody Anti-Mouse IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
1/25000.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| Immunodiffusion |
Use at an assay dependent concentration.
|
|
| Conjugation |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| WB |
1/3000 - 1/15000.
|
| 说明 |
|---|
|
ELISA
1/25000. |
|
IP
Use at an assay dependent concentration. |
|
Immunodiffusion
Use at an assay dependent concentration. |
|
Conjugation
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
WB
1/3000 - 1/15000. |
图片
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Western blot - Goat Anti-Mouse IgG H&L (ab6708)Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution + Mouse IgG at 0.1 µg
Secondary
Secondary antibody: DyLight™ 800 goat secondary antibody at 1/20000 dilution
Observed band size: 28,55 kDa why is the actual band size different from the predicted?ab6708 incubation overnight at 4°C.
Both heavy (55kDa) and light (28kDa) chains are detected.
-
Western blot - Goat Anti-Mouse IgG H&L (ab6708)All lanes : Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution
Lane 1 : Mouse IgG (whole molecule)
Lane 2 : Mouse IgG Fc fragment
Lane 3 : Mouse IgG Fab fragment
Lane 4 : Mouse IgM kappa
Lane 5 : Mouse serum
Lysates/proteins at 0.05 µg per lane.
Secondary
All lanes : HRP Donkey Anti-Goat IgG at 1/40000 dilutionab6708 incubated for 1 hour at room temperature.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (63)
ab6708 被引用在 63 文献中.
- Lee S et al. Oomycete effector AVRblb2 targets cyclic nucleotide-gated channels through calcium sensors to suppress pattern-triggered immunity. New Phytol 241:1277-1291 (2024). PubMed: 38013595
- Verschoor YL et al. Neoadjuvant atezolizumab plus chemotherapy in gastric and gastroesophageal junction adenocarcinoma: the phase 2 PANDA trial. Nat Med 30:519-530 (2024). PubMed: 38191613
- Yick LW et al. T follicular helper cells contribute to pathophysiology in a model of neuromyelitis optica spectrum disorders. JCI Insight 8:N/A (2023). PubMed: 36649074
- Li Y et al. Tumor Cell Nanovaccines Based on Genetically Engineered Antibody-Anchored Membrane. Adv Mater 35:e2208923 (2023). PubMed: 36715052
- Li T et al. Nanoparticle-mediated TRPV1 channel blockade amplifies cancer thermo-immunotherapy via heat shock factor 1 modulation. Nat Commun 14:2498 (2023). PubMed: 37120615