Goat Anti-Mouse IgG H&L (HRP)

• WB

Unknown

Western blot - Goat Anti-Mouse IgG H&L (HRP) (AB6789)

All lanes:

Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (<a href='/products/primary-antibodies/gapdh-antibody-mabcam-9484-loading-control-ab9484'>ab9484</a>) at 0.5 µg/mL

All lanes:

HeLa cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/5000 dilution

Predicted band size: 36 kDa

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Exposure time: 30s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (AB6789)

IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.

The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8224, 3 μg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

The inset negative control image is secondary-only at 1/500 dilution.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• ELISA

Supplier Data

ELISA - Goat Anti-Mouse IgG H&L (HRP) (AB6789)

ELISA results of ab6789 against purified Mouse IgG protein. Each well was coated in duplicate with 10 µg of Mouse IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1/106,000. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

• WB

AbReview28802****

Western blot - Goat Anti-Mouse IgG H&L (HRP) (AB6789)

All lanes:

Anti-CDC42 Binding Protein Kinase Beta antibody (<a href='/products/unavailable/cdc42-binding-protein-kinase-beta-antibody-ab61328'>ab61328</a>) at 1/2000 dilution

All lanes:

Cytosolic fraction prepared from murine brain tissue at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/2000 dilution

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Exposure time: 1min

This image is courtesy of an anonymous Abreview.

• IP

AbReview37027****

Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (AB6789)

ab49969 Immunoprecipitating HA Tag in HEK-293T whole cell lysate. 25 μg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.

Secondary

All lanes:

Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)

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This image is courtesy of an anonymous Abreview.

• WB

Unknown

Western blot - Goat Anti-Mouse IgG H&L (HRP) (AB6789)

ab6789 was used at dilution 1/10000 with the primary antibody ab55504 in WB. See the review on ab55504.

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/10000 dilution

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• WB

Supplier Data

Western blot - Goat Anti-Mouse IgG H&L (HRP) (AB6789)

Western blot analysis of ab6789 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Mouse IgG at 50 ng

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/1000 dilution

false