山羊抗人IgM H&L (FITC) (ab8497)
Key features and details
- Goat Anti-Human IgM H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Suitable for: ICC/IF
概述
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产品名称
山羊抗人IgM H&L (FITC)
参阅全部 IgM 二抗 -
宿主
Goat -
靶标种属
Human -
经测试应用
适用于: ICC/IFmore details -
偶联物
FITC. Ex: 493nm, Em: 528nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol, 0.58% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgM. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgM a minimum of 65% protein bound. -
共轭说明
The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. -
克隆
多克隆 -
轻链类型
unknown -
常规说明
F/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgM. There is low background fluorescence in normal tissue in contrast to cases of auto immune desease where IgM is depostited. -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
| 说明 |
|---|
应用说明
For immunohistochemical staining of human tissue sections a 1:40 dilution is recommended.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab8497 尚未被引用在任何文献中。