山羊抗人IgG H&L (HRP) (ab81202)
Key features and details
- Goat Anti-Human IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ELISA
概述
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产品名称
山羊抗人IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Human -
特异性
No crossreaction with human IgM and IgA in ELISA No crossreaction with albumin fraction of human serum. No crossreaction with albumin fraction of other animal species. -
经测试应用
适用于: WB, ELISAmore details -
免疫原
Human IgG.
-
偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.50
Constituents: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound. -
共轭说明
The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel filtation and ion exchange chromatography to clear unbound conjugate. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81202于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/100.
|
|
| ELISA |
1/1000.
|
| 说明 |
|---|
|
WB
1/100. |
|
ELISA
1/1000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab81202 被引用在 5 文献中.
- Seton KA et al. Investigating Antibody Reactivity to the Intestinal Microbiome in Severe Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): A Feasibility Study. Int J Mol Sci 24:N/A (2023). PubMed: 37895005
- Kazmierczak Z et al. Immune Response to Therapeutic Staphylococcal Bacteriophages in Mammals: Kinetics of Induction, Immunogenic Structural Proteins, Natural and Induced Antibodies. Front Immunol 12:639570 (2021). PubMed: 34194425
- Karaaslan E et al. Immune responses in multiple hosts to Nucleocapsid Protein (NP) of Crimean-Congo Hemorrhagic Fever Virus (CCHFV). PLoS Negl Trop Dis 15:e0009973 (2021). PubMed: 34851958
- Maldonado-Ruiz LP et al. Differential Tick Salivary Protein Profiles and Human Immune Responses to Lone Star Ticks (Amblyomma americanum) From the Wild vs. a Laboratory Colony. Front Immunol 10:1996 (2019). PubMed: 31555263
- Londono-Renteria BL et al. Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Proteins in Colombia. Front Public Health 6:111 (2018). PubMed: 29868532