山羊抗人IgG H&L (Biotin)预吸附二抗(ab7152)
Key features and details
- Goat Anti-Human IgG H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Goat
- Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, Immunomicroscopy, WB
概述
-
产品名称
山羊抗人IgG H&L (Biotin)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Human -
特异性
No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
-
经测试应用
适用于: ICC/IF, Dot blot, ELISA, IHC-P, Immunomicroscopy, WBmore details -
最小交叉反应
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details -
免疫原
Full length native human IgG (purified).
-
偶联物
Biotin
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Secondary antibody conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule -
克隆
多克隆 -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ELISA |
1/200000.
|
|
| IHC-P |
1/500 - 1/2500.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| WB |
1/2000 - 1/10000.
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
Dot blot
Use at an assay dependent concentration. |
|
ELISA
1/200000. |
|
IHC-P
1/500 - 1/2500. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
WB
1/2000 - 1/10000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (4)
ab7152 被引用在 4 文献中.
- Zhang YN et al. Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimmers as HIV-1 vaccine candidates. Nat Commun 14:1985 (2023). PubMed: 37031217
- Zhang YN et al. Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants. bioRxiv N/A:N/A (2021). PubMed: 33791704
- Zhang YN et al. Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants. Sci Adv 7:eabj3107 (2021). PubMed: 34669468
- Limberis MP et al. Intranasal antibody gene transfer in mice and ferrets elicits broad protection against pandemic influenza. Sci Transl Med 5:187ra72 (2013). Human . PubMed: 23720583
