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山羊抗豚鼠IgG H&L (Texas Red ®) (ab6906)

Submit a review Q&A (4)References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Guinea pig IgG H&L (Texas Red ®) (ab6906)

    Key features and details

    • Goat Anti-Guinea pig IgG H&L (Texas Red ®)
    • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
    • Host species: Goat
    • Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISA

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase Biotin Cy3 ® Cy3.5 ® Cy5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm HRP TRITC Unconjugated

    概述

    • 产品名称

      山羊抗豚鼠IgG H&L (Texas Red ®)
      参阅全部 IgG 二抗

    • 宿主

      Goat

    • 靶标种属

      Guinea pig

    • 经测试应用

      适用于: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details

    • 免疫原

      Full length native Guinea Pig IgG (purified).

    • 偶联物

      Texas Red ®. Ex: 596nm, Em: 620nm

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
    • Concentration information loading...

    • 纯度

      Affinity purified

    • 纯化说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • 共轭说明

      Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG

    • 克隆

      多克隆

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6906于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF
    IHC-P
    IHC-Fr
    Immunomicroscopy
    Flow Cyt
    ELISA
    说明
    应用说明
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Guinea pig IgG H&L (Texas Red ®) (ab6906)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Guinea pig IgG H&L (Texas Red ®) (ab6906)Image from Arriola DJ et al., J Biol Chem. 2012 Sep 28;287(40):33424-35. Epub 2012 Aug 3. Fig 2.; doi: 10.1074/jbc.M112.362103; September 28, 2012, The Journal of Biological Chemistry, 287, 33424-33435.
      Immunohistochemical analysis of mouse pituitary tissue. Tissue was stained with a guinea pig primary antibody before staining with secondary antibody (ab6906 at 1/300 dilution) for 60 minutes at room temperature.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (5)

    发表研究结果有使用 ab6906？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6906 被引用在 5 文献中.

    • Katsumoto K  et al. Wnt4 is heterogeneously activated in maturing β-cells to control calcium signaling, metabolism and function. Nat Commun 13:6255 (2022). PubMed: 36271049
    • Ilie I  et al. Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway. Int J Nanomedicine 8:3345-53 (2013). ICC, Flow Cyt . PubMed: 24039418
    • Arriola DJ  et al. FOXO1 Transcription Factor Inhibits Luteinizing Hormone ß Gene Expression in Pituitary Gonadotrope Cells. J Biol Chem 287:33424-35 (2012). IHC-P . PubMed: 22865884
    • Noda K  et al. An animal model of spontaneous metabolic syndrome: Nile grass rat. FASEB J 24:2443-53 (2010). IHC . PubMed: 20335226
    • Huang Y  et al. Bone marrow transplantation temporarily improves pancreatic function in streptozotocin-induced diabetes: potential involvement of very small embryonic-like cells. Transplantation 89:677-85 (2010). IHC-Fr . PubMed: 20110858

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Question

    Thank you so much for your reply. Yes, I did washing with 1xPBS 3 times after fixing, after permeablization and after primary antibody incubation. If I can, I'd like to replace this product with product ab7137 which is pre-absorbed so that the chances to get background may be lower.

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    Answer

    I have arranged a free replacement vial of ab7137 to be sent to you on order 161903 today. I hope you will be satisfied with this product.

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    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER ER52866 DESCRIPTION OF THE PROBLEM high background even without primary antibody SAMPLE human epidermal cells: keratinocytes PRIMARY ANTIBODY ab7842: Guinea pig polyclonal to insulin DETECTION METHOD Nikon microscope ANTIBODY STORAGE CONDITIONS just received the day before, and store at 4 centigrade FIXATION OF SAMPLE 4% paraformaldehyde 15 minutes ANTIGEN RETRIEVAL No PERMEABILIZATION STEP 0.25% Triton-x100 10minutes BLOCKING CONDITIONS 1%BSA in PBS for 30minutes SECONDARY ANTIBODY ab6906: Goat polyclonal to Guinea Pig IgG H&L (texas red) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? I tried different dilution rate: 1:200, 1:1000, 1:2000, I also tried different block solution, 1%BSA, 5%BSA, 1%milk, 5%milk, and 1%BSA with 5% goat serum

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    Answer

    I'm very sorry to hear you are having problems with ab6906. The fact that a no primary test gave you high background is very concerning and it certainly suggests the vial you received was damaged during shipping or storage. I looked at the tracking information of your order and it seemed that the package was delayed as fedex have recorded "Customer not available or business closed" and delivered the parcel at another address first. Can I please verify that you wash in PBS several times in between steps to remove the paraformaldehyde and primary antibody? Can you please tell if if you would like a refund or a replacement? I look forward to hearing from you,

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    Question

    Is this antibody tested for reactivity against other species IgG as ab7137? Sincerely

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    Answer

    Thank you for your enquiry. This antibody has not been preabsorbed against other species IgG. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    I have a question about the conjugation method used to conjugate the Texas Red to the antibodies. I'm doing a secondary conjugation and for some reason they only want to attach to the antibodies attached to the Texas Red, so I'd like to know the linker used.

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    Answer

    The Texas Red we use for the conjugation is Texas Red® sulfonyl chloride. The active functional group of the dye reacts with a primary amine of the antibody and forms a covalent bond. If the secondary conjugation is targeting the amine or carboxyl or sulfhydral groups, I am not surprised that it only wants to attach to the antibody. I hope this information helps. If you have any additional questions, please contact us again.

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