山羊F(ab')2 Anti-大鼠IgG - H&L (HRP),预吸附二抗(ab6120)
Key features and details
- Goat F(ab')2 Anti- Rat IgG - H&L (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC
概述
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产品名称
山羊F(ab')2 Anti-大鼠IgG - H&L (HRP),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rat -
特异性
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
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经测试应用
适用于: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
最小交叉反应
Cow, Horse, Human, Mouse, Rabbit, Sheep more details -
免疫原
Rat IgG whole molecule
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Substrate reagent
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- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | ||
| Dot blot | ||
| WB | ||
| ELISA | ||
| Electron Microscopy | ||
| IHC |
| 说明 |
|---|
应用说明
Dot: Use at an assay dependent dilution.
Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/4000 - 1/16000.
IHC: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4000 to 1/16000 of the reconstitution concentration is suggested for this product.
Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/4000 - 1/16000.
IHC: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4000 to 1/16000 of the reconstitution concentration is suggested for this product.
Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (5)
ab6120 被引用在 5 文献中.
- Chen J et al. Patient-Derived Xenografts Are a Reliable Preclinical Model for the Personalized Treatment of Epithelial Ovarian Cancer. Front Oncol 11:744256 (2021). PubMed: 34671560
- Singh A et al. Hippo Signaling Mediators Yap and Taz Are Required in the Epicardium for Coronary Vasculature Development. Cell Rep 15:1384-1393 (2016). PubMed: 27160901
- Leng X et al. Evidence of a role for both anti-Hsp70 antibody and endothelial surface membrane Hsp70 in atherosclerosis. Cell Stress Chaperones 18:483-93 (2013). PubMed: 23334859
- Singh MK et al. MicroRNA-processing Enzyme Dicer Is Required in Epicardium for Coronary Vasculature Development. J Biol Chem 286:41036-45 (2011). IHC-Fr . PubMed: 21969379
- Leng X et al. Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats. Cell Stress Chaperones 15:947-58 (2010). ELISA . PubMed: 20607472
