山羊F(ab')2抗大鼠IgG - Fc (PE),预吸附二抗(ab6259)
Key features and details
- Goat F(ab')2 Anti-Rat IgG - Fc (PE), pre-adsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt
Related conjugates and formulations
概述
-
产品名称
山羊F(ab')2抗大鼠IgG - Fc (PE),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rat -
特异性
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab) or Bovine, Horse and Human Serum Proteins.
-
经测试应用
适用于: ICC/IF, Immunomicroscopy, Flow Cytmore details -
最小交叉反应
Cow, Horse, Human more details -
免疫原
Rat IgG F(c) fragment
-
偶联物
PE. Ex: 488nm, Em: 575nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
BSA is IgG and protease free -
Concentration information loading...
-
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound) -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100 - 1/250.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
|
Flow Cyt |
Use at an assay dependent dilution.
|
说明 |
---|
ICC/IF
1/100 - 1/250. |
Immunomicroscopy
Use at an assay dependent dilution. |
Flow Cyt
Use at an assay dependent dilution. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab6259 被引用在 1 文献中.
- Mutoh T et al. Identification of two major autoantigens negatively regulating endothelial activation in Takayasu arteritis. Nat Commun 11:1253 (2020). PubMed: 32152303