山羊F(ab')2抗大鼠IgG - Fc (HRP),预吸附二抗(ab6257)
Key features and details
- Goat F(ab')2 Anti-Rat IgG - Fc (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Electron Microscopy
Related conjugates and formulations
概述
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产品名称
山羊F(ab')2抗大鼠IgG - Fc (HRP),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rat -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab’)2 or Bovine, Horse, Human Serum Proteins. -
经测试应用
适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Electron Microscopymore details -
最小交叉反应
Cow, Horse, Human more details -
免疫原
Rat IgG F(c) whole molecule
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free. Warning: use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase. -
Concentration information loading... -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | ||
| Dot blot | ||
| WB | ||
| ELISA | ||
| IHC | ||
| Electron Microscopy |
| 说明 |
|---|
应用说明
Dot: Use at an assay dependent dilution.
Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/20000 - 1/200000.
IHC: 1/500 - 1/5000.
WB: 1/20000 - 1/200000.
This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20000 to 1/200000 of the reconstitution concentration is suggested for this product for ELISA and Western blots and a 1/500 to 1/5000 for immunohistochemistry on tissue sections.
Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/20000 - 1/200000.
IHC: 1/500 - 1/5000.
WB: 1/20000 - 1/200000.
This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20000 to 1/200000 of the reconstitution concentration is suggested for this product for ELISA and Western blots and a 1/500 to 1/5000 for immunohistochemistry on tissue sections.
Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab6257 被引用在 3 文献中.
- Xiao Y et al. An oligodeoxynucleotide with AAAG repeats significantly attenuates burn-induced systemic inflammatory responses via inhibiting interferon regulatory factor 5 pathway. Mol Med 23:N/A (2017). PubMed: 28620671
- Matrasova I et al. Heterogeneity of molecular forms of dipeptidyl peptidase-IV and fibroblast activation protein in human glioblastomas. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repu 161:252-260 (2017). PubMed: 28452380
- Gomez AM et al. Proteasome inhibition with bortezomib depletes plasma cells and autoantibodies in experimental autoimmune myasthenia gravis. J Immunol 186:2503-13 (2011). Sandwich ELISA . PubMed: 21239719