山羊F(ab')2抗大鼠IgG - (Fab)'2 (TR),预吸附二抗(ab6267)
Key features and details
- Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ELISA, Immunomicroscopy, Flow Cyt
Related conjugates and formulations
概述
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产品名称
山羊F(ab')2抗大鼠IgG - (Fab)'2 (TR),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rat -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins. -
经测试应用
适用于: ICC/IF, ELISA, Immunomicroscopy, Flow Cytmore details -
最小交叉反应
Cow, Horse, Human more details -
免疫原
Rat IgG F(ab’)2 fragment
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偶联物
Texas Red ®. Ex: 596nm, Em: 620nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.5
Preservative: 0.05% Sodium azide
Constituents: 1.46% Sodium chloride, 1% BSA, 0.164% Sodium phosphate
BSA is IgG and protease free -
Concentration information loading... -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.5 moles Texas Red per mole of Goat IgG F(ab’)2 -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | ||
| ELISA | ||
| Immunomicroscopy | ||
| Flow Cyt |
| 说明 |
|---|
应用说明
Flow Cyt: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab6267 被引用在 14 文献中.
- Qi JL et al. SQSTM1/p62 regulate breast cancer progression and metastasis by inducing cell cycle arrest and regulating immune cell infiltration. Genes Dis 9:1332-1344 (2022). PubMed: 35873020
- Shepherdson JL et al. Delineation of the 1q24.3 microdeletion syndrome provides further evidence for the potential role of non-coding RNAs in regulating the skeletal phenotype. Bone 142:115705 (2021). PubMed: 33141070
- Wang S et al. Relationship between long non-coding RNA PCAT-1 expression and gefitinib resistance in non-small-cell lung cancer cells. Respir Res 22:146 (2021). PubMed: 33980216
- Zheng H et al. Expression profiling of mitochondria-associated microRNAs during osteogenic differentiation of human MSCs. Bone 151:116058 (2021). PubMed: 34144232
- Xiao H et al. Metformin ameliorates bleomycin-induced pulmonary fibrosis in mice by suppressing IGF-1. Am J Transl Res 12:940-949 (2020). PubMed: 32269725