山羊F(ab')2抗大鼠IgG - (Fab')2 (HRP),预吸附二抗(ab6517)
Key features and details
- Goat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC
Related conjugates and formulations
概述
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产品名称
山羊F(ab')2抗大鼠IgG - (Fab')2 (HRP),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rat -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins. -
经测试应用
适用于: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
最小交叉反应
Cow, Horse, Human more details -
免疫原
Rat IgG F(ab’)2 fragment
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偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.16% Sodium phosphate
BSA is IgG and protease free -
Concentration information loading... -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/5000.
|
|
| Dot blot |
Use at an assay dependent dilution.
|
|
| WB |
Use at an assay dependent dilution.
|
|
| ELISA |
1/10000 - 1/100000.
|
|
| Electron Microscopy |
Use at an assay dependent dilution.
|
|
| IHC |
Use at an assay dependent dilution.
|
| 说明 |
|---|
|
ICC/IF
1/500 - 1/5000. |
|
Dot blot
Use at an assay dependent dilution. |
|
WB
Use at an assay dependent dilution. |
|
ELISA
1/10000 - 1/100000. |
|
Electron Microscopy
Use at an assay dependent dilution. |
|
IHC
Use at an assay dependent dilution. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (3)
ab6517 被引用在 3 文献中.
- Motomura E et al. Cell-autonomous signal transduction in the Xenopus egg Wnt/ß-catenin pathway. Dev Growth Differ 56:640-52 (2014). WB . PubMed: 25330272
- Wang SH et al. Advanced mass spectrometry and chemical analyses reveal the presence of terminal disialyl motif on mouse B-cell glycoproteins. Glycobiology N/A:N/A (2013). PubMed: 23363740
- Engelson EJ et al. An Essential Farnesylated Kinesin in Trypanosoma brucei. PLoS One 6:e26508 (2011). WB . PubMed: 22073170
