山羊F(ab')2抗大鼠IgG - (Fab')2 (FITC),预吸附二抗(ab6266)
Key features and details
- Goat F(ab')2 Anti-Rat IgG - (Fab')2 (FITC), pre-adsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISA
Related conjugates and formulations
概述
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产品名称
山羊F(ab')2抗大鼠IgG - (Fab')2 (FITC),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rat -
特异性
No reaction was observed against Rat IgG F(c), anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse and Human Serum Proteins.
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经测试应用
适用于: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details -
最小交叉反应
Cow, Horse, Human more details -
免疫原
Rat IgG F(ab’)2 fragment
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偶联物
FITC. Ex: 493nm, Em: 528nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.164% Sodium phosphate -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.1 moles FITC per mole of Goat IgG F(ab’)2 -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
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| Flow Cyt |
Use at an assay dependent dilution.
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|
| Immunomicroscopy |
Use at an assay dependent dilution.
|
|
| ELISA |
1/10000 - 1/50000.
|
| 说明 |
|---|
|
ICC/IF
1/1000 - 1/5000. |
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Flow Cyt
Use at an assay dependent dilution. |
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Immunomicroscopy
Use at an assay dependent dilution. |
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ELISA
1/10000 - 1/50000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab6266 被引用在 2 文献中.
- Ufimtseva E Differences between Mycobacterium-Host Cell Relationships in Latent Tuberculous Infection of Mice Ex Vivo and Mycobacterial Infection of Mouse Cells In Vitro. J Immunol Res 2016:4325646 (2016). PubMed: 27066505
- Ufimtseva E Mycobacterium-Host Cell Relationships in Granulomatous Lesions in a Mouse Model of Latent Tuberculous Infection. Biomed Res Int 2015:948131 (2015). PubMed: 26064970