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山羊F(ab')2抗大鼠IgG - (Fab')2 (Biotin),预吸附二抗(ab6275)

Submit a review Q&A (1)References (3)

Key features and details

  • Goat F(ab')2 Anti-Rat IgG - (Fab')2 (Biotin), pre-adsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy

Conjugates logo Related conjugates and formulations

Alkaline Phosphatase FITC HRP PE Texas Red ® Unconjugated

概述

  • 产品名称

    山羊F(ab')2抗大鼠IgG - (Fab')2 (Biotin),预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Rat

  • 特异性

    No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.

  • 经测试应用

    适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details

  • 最小交叉反应


    Cow, Horse, Human more details

  • 免疫原

    Rat IgG F(ab')2 fragment

  • 偶联物

    Biotin

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 1.46% Sodium chloride, 1.5% BSA, 0.164% Sodium phosphate

    BSA is IgG and protease free
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • 共轭说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6275于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/500 - 1/5000.
Dot blot
Use at an assay dependent dilution.
WB
1/2000 - 1/10000.
ELISA
1/20000 - 1/400000.
IHC
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
说明
ICC/IF
1/500 - 1/5000.
Dot blot
Use at an assay dependent dilution.
WB
1/2000 - 1/10000.
ELISA
1/20000 - 1/400000.
IHC
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (3)

发表研究结果有使用 ab6275？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6275 被引用在 3 文献中.

  • Liu B  et al. The overexpression of RBM3 alleviates TBI-induced behaviour impairment and AD-like tauopathy in mice. J Cell Mol Med 24:9176-9188 (2020). PubMed: 32648620
  • Wu X  et al. Hydrogen exerts neuroprotective effects on OGD/R damaged neurons in rat hippocampal by protecting mitochondrial function via regulating mitophagy mediated by PINK1/Parkin signaling pathway. Brain Res 1698:89-98 (2018). PubMed: 29958907
  • Mochida GH  et al. CHMP1A encodes an essential regulator of BMI1-INK4A in cerebellar development. Nat Genet 44:1260-4 (2012). PubMed: 23023333

客户评价及客户问答

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Question

I need a anti-rat biotin conjugated secondary Ab raised in goat to perform IHC on human joint tissue. In addition to ab6275, you have ab6619, 6256, 7176 in your catalog that match that description. Can you tell me if there is any difference between these antibodies and which one you recommend for this application (IHC)?

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Answer

Thank you for your enquiry. Ab6275: Biotin Conjugated F(ab')2 Fragment of Affinity Purified anti-Rat IgG F(ab')2 [Goat] Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins - This product is the F(ab')2 portion of the goat IgG that is raised against Rat F(ab')2. Ab7176: Biotin Conjugated Fab Fragment of Affinity Purified anti-Rat IgG [H&L] [Goat] - This product is the Fab portion (single Fab fragments) of the Goat IgG raised against Rat IgG. Ab6256:Biotin Conjugated F(ab')2 Fragment of Affinity Purified anti-Rat IgG F(c) [Goat] Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins - This product is the F(ab')2 portion of the goat IgG that is raised against Rat F(c). Typically Fab and Fab2 fragments will bind less than the entire IgG molecule. This means these types of products are good for assays in which very low background is desired. I hope I was able to make things more clear. Please do not hesitate to contact me if you have any other questions regarding this issue.

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