山羊F(ab')2抗大鼠IgG - (Fab)'2 (AP),预吸附二抗(ab6518)
Key features and details
- Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (AP), pre-adsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, IHC, ICC/IF, Dot blot, WB
Related conjugates and formulations
概述
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产品名称
山羊F(ab')2抗大鼠IgG - (Fab)'2 (AP),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rat -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG F(ab)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat Fc or Bovine, Horse, and Human Serum Proteins -
经测试应用
适用于: ELISA, IHC, ICC/IF, Dot blot, WBmore details -
最小交叉反应
Cow, Horse, Human more details -
免疫原
Rat IgG F(ab)2 fragment
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偶联物
Alkaline Phosphatase
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 1.5% BSA, 0.01% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol (glycerin, glycerine)
BSA is IgG and protease free -
Concentration information loading... -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
1/5000 - 1/50000.
|
|
| IHC |
Use at an assay dependent dilution.
|
|
| ICC/IF |
1/200 - 1/1000.
|
|
| Dot blot |
Use at an assay dependent dilution.
|
|
| WB |
1/5000 - 1/50000.
|
| 说明 |
|---|
|
ELISA
1/5000 - 1/50000. |
|
IHC
Use at an assay dependent dilution. |
|
ICC/IF
1/200 - 1/1000. |
|
Dot blot
Use at an assay dependent dilution. |
|
WB
1/5000 - 1/50000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab6518 尚未被引用在任何文献中。