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山羊F(ab')2抗兔IgG - H&L (Biotin),预吸附二抗(ab6012)

Submit a review Q&A (1)References (12)

Key features and details

  • Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, IHC, Immunomicroscopy

Conjugates logo Related conjugates and formulations

Alkaline Phosphatase DyLight® 488 DyLight® 550 FITC HRP Unconjugated

概述

  • 产品名称

    山羊F(ab')2抗兔IgG - H&L (Biotin),预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Rabbit

  • 特异性

    No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

  • 经测试应用

    适用于: ICC/IF, IHC-P, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details

  • 最小交叉反应


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • 免疫原

    Rabbit IgG whole molecule

  • 偶联物

    Biotin

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 6.50
    Preservative: 0.01% Sodium azide
    Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • 共轭说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6012于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent dilution. PubMed: 21498392
Dot blot
Use at an assay dependent dilution.
WB
1/2000 - 1/10000.
ELISA
1/20000 - 1/100000.
IHC
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
说明
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent dilution. PubMed: 21498392
Dot blot
Use at an assay dependent dilution.
WB
1/2000 - 1/10000.
ELISA
1/20000 - 1/100000.
IHC
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (12)

发表研究结果有使用 ab6012？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6012 被引用在 12 文献中.

  • Schülke LC  et al. Identification of two hidden clinical subgroups among men with idiopathic cryptozoospermia. Hum Reprod 39:892-901 (2024). PubMed: 38365879
  • Siebert-Kuss LM  et al. Transcriptome analyses in infertile men reveal germ cell-specific expression and splicing patterns. Life Sci Alliance 6:N/A (2023). PubMed: 36446526
  • Hiort M  et al. Testicular Architecture of Men with 46,XX Testicular Disorders of Sex Development. Sex Dev 17:32-42 (2023). PubMed: 36746123
  • Di Persio S  et al. Single-cell RNA-seq unravels alterations of the human spermatogonial stem cell compartment in patients with impaired spermatogenesis. Cell Rep Med 2:100395 (2021). PubMed: 34622232
  • Silva AF  et al. Effects of chronic moderate alcohol consumption on right ventricle and pulmonary remodelling. Exp Physiol 106:1359-1372 (2021). PubMed: 33605491
View all Publications for this product

客户评价及客户问答

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Question

How about if this antibody ab8888 (anti-Dopamine) conduct for mouse brain sample IHC experiment?
Besides, customer consider use monoclonal antibody such as ab8892(anti-Dopamine) use for mouse sample IHC.
Customer concern cross reaction of secondary anibody.
Which secondary antibody you suggest since their brain sample also from mouse.
Thanks a lot for your assistance.
Best regards,

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Answer

Thank you for contacting us.

If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse species to avoid cross reactivity.

We have some secondaries in our catalogue raised in Goat that could be appropriate for your assay:

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-ab-2-F-ab-2-Fragment-ab6112.html

https://www.abcam.com/Goat-F-ab-2-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Biotin-pre-adsorbed-ab6012.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-c-F-ab-2-Fragment-ab6021.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-F-ab-2-Fragment-ab6013.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Fab-Fragment-ab7171.html

It is also very important to use a blocking agent such as serum from the same species as the secondary was raised in. If you used any of the secondaries suggested, therefore, 10% goat serum would be the most suitable blocking agent.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information

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