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山羊F(ab')2抗兔IgG Fc (Biotin) (ab6021)

Submit a review Q&A (1)References (1)

Key features and details

  • Goat F(ab')2 Anti-Rabbit IgG Fc (Biotin)
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy, IP

Conjugates logo Related conjugates and formulations

Alkaline Phosphatase FITC TRITC Unconjugated

概述

  • 产品名称

    山羊F(ab')2抗兔IgG Fc (Biotin)
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Rabbit

  • 特异性

    No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Rabbit IgG F(ab).

  • 经测试应用

    适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy, IPmore details

  • 免疫原

    Rabbit IgG F(c) fragment

  • 偶联物

    Biotin

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 6.50
    Preservative: 0.01% Sodium azide
    Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    F(ab') fragment biotin conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • 共轭说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab’)2 molecule

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6021于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent dilution.
WB
1/2000 - 1/10000.
ELISA
1/20000 - 1/100000.
IHC
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
IP
Use at an assay dependent dilution.
说明
ICC/IF
1/1000 - 1/5000.
Dot blot
Use at an assay dependent dilution.
WB
1/2000 - 1/10000.
ELISA
1/20000 - 1/100000.
IHC
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
IP
Use at an assay dependent dilution.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab6021？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6021 被引用在 1 文献中.

  • Lee JH  et al. 53BP1 promotes ATM activity through direct interactions with the MRN complex. EMBO J : (2009). IP . PubMed: 20010693

客户评价及客户问答

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Question

How about if this antibody ab8888 (anti-Dopamine) conduct for mouse brain sample IHC experiment?
Besides, customer consider use monoclonal antibody such as ab8892(anti-Dopamine) use for mouse sample IHC.
Customer concern cross reaction of secondary anibody.
Which secondary antibody you suggest since their brain sample also from mouse.
Thanks a lot for your assistance.
Best regards,

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Answer

Thank you for contacting us.

If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse species to avoid cross reactivity.

We have some secondaries in our catalogue raised in Goat that could be appropriate for your assay:

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-ab-2-F-ab-2-Fragment-ab6112.html

https://www.abcam.com/Goat-F-ab-2-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Biotin-pre-adsorbed-ab6012.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-c-F-ab-2-Fragment-ab6021.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-F-ab-2-Fragment-ab6013.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Fab-Fragment-ab7171.html

It is also very important to use a blocking agent such as serum from the same species as the secondary was raised in. If you used any of the secondaries suggested, therefore, 10% goat serum would be the most suitable blocking agent.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information

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